目的 研究不明原因反复流产患者蜕膜组织CD4、CD8免疫细胞的局部表达及与TM、PAI-1、F1+2、D-二聚体等凝血相关因子的关联性,为不明原因流产患者治疗提供帮助.方法 采用细胞免疫芯片技术对不明原因流产患者50例子宫蜕膜组织进行CD4、CD8细胞计数并计算CD4/CD8比值;酶联免疫吸附试验方法测定患者血浆血栓调节蛋白(TM)、纤溶酶原激活抑制物-1(PAI-1)、凝血酶原片段F1+2;全自动免疫荧光分析法测定D二聚体(D-dimer,D-D)因子浓度.同时以正常妊娠无流产征兆主动要求人工流产的50人为对照组,比较上述指标在两个群体中的表达,并进行对比分析研究.结果 与对照组比较,患者蜕膜组织CD4水平及CD4/CD8比值均下降,CD8水平呈高表达,血浆TM、PAI-1、F1+2因子呈高表达,D-D呈低表达状态.两组比较差异均有统计学意义(P＜0.05).结论 反复流产患者蜕膜组织局部免疫功能表达异常,血液处于一定程度的高凝状态而纤溶系统受损.修复上述状态可对治疗不明原因反复流产患者提供新思路.%Objective To study the local expression of membrane tissue CD4,CD8 immune cells and TM,PAI-1,F1 + 2,D-dimers coagulation related factors in unexplained recurrent spontaneous abortion patients and their relevance,so to provide help for the treatment of patients with unexplained abortion.Methods The CD4/CD8 cells were counted and the CD4/CD8 ratio was calculated by immunocytochemistry.Using enzymelinked immunosorbent assay and adsorption test method in 50 patients with unexplained abortion (experimental group,non-pregnant) for plasma thrombomodulin (TM),plasminogen activator inhibitor-1 (PAI-1),prothrombin fragment F1+2,D dimer (D-dimer,D-D) and the normal pregnancy factor concentration,and 50 women (control group) as control,the expression of the above two groups factors in two groups were measured,and then compared the results.Results Compared with the control group,TM,PAI-1 and F1 +2 were highly expressed,and the expression of D-D was low.The difference between the two groups was statistically significant (P ＜ 0.05).Conclusion The blood of patients with recurrent abortion was in a state of high coagulation and the fibrinolytic system was damaged.Restoration of the above status provides new ideas for the treatment of unknown causes.
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