微波技术在黄花菜多糖提取中的应用

摘要

研究应用微波技术以黄花菜为原料提取黄花菜多糖的新工艺。在单因素实验的基础上，通过正交实验确定最佳提取工艺参数。结果表明，黄花菜多糖最佳提取工艺参数为微波功率490 W，提取时间100 s，液固比25∶1，提取2次。按此工艺参数提取，多糖平均得率为31．65％，RSD＝2．21％（n＝3）；多糖平均含量为18．89％，RSD＝2．63％（n＝3）；平均回收率为97．92％，RSD＝3．13％（n＝6）。与水提醇沉法相比，应用微波技术提取黄花菜多糖，每次提取的时间由3 h减少到100 s，而多糖含量提高了23．63％。%A new method of microwave technique was used to extract the polysaccharides from Daylily.Based on the results of single factor tests,the optimum parameters of polysaccharides extraction were studied by orthogonal tests.The results showed that the optimal microwave power,the time for extraction the ratio of liquid to solid and times of extraction,were 490W,100s,25 ∶1 and 2 times,respectively.With the techniques,the average rate of polysaccharides extraction was 31.65%,RSD=2.21%(n=3),the average content of polysaccharides 18.89%, RSD=2.63%(n=3),and the average recovery rate 97.92%,RSD=3.13%(n=6).Compared with water ex-traction and alcohol precipitation,the time for extraction with microwave was reduced from 3 hours to 100 s and the content of polysaccharides was increased 23 .63%.