河南省耐多药结核分枝杆菌利福平耐药基因rpoB的突变特征

摘要

Aim:To analyze the characteristics of the mutations of rpoB genes in multidrug resistant Mycobacterium tuberculosis ( MDR-TB ) clinical isolates from Henan province. Methods: A total of 150 MDR-TB clinical strains were collected and the mutation patterns of rpoB full ORF was detected by PCR and sequencing with H37Rv as control. Results: 97. 2% ( 140/144 ) isolates carried mutations in the full ORF of rpoB gene, and 96. 5%( 139/144 ) isolates carried mutations in RRDR. 99% ( 139/140 ) isolates showed mutations both in the RRDR and outside the RRDR. The top 3 loci with high mutation frequency were rpoB531, rpoB526 and rpoB516. Discovered 15 mutation loci and 4 mutation types that had not been recorded previously. Among them, rpoB1284 probably was polymorphism locus and had nothing to do with rifamp-cin resistance. Conclusion: It is of high sensitivity to discover MDR-TB by detecting mutation loci within RRDR.%目的:分析河南省耐多药(MDR)结核分枝杆菌利福平耐药相关基因rpoB的突变特征.方法:收集150株MDR结核分枝杆菌,应用PCR扩增利福平耐药相关基因rpoB编码区全长并测序,以结核分枝杆菌H37Rv标准株DNA序列为参比序列,分析菌株的突变特征.结果:MDR菌株rpoB基因编码区存在突变者占97.2%(140/144),突变在利福平耐药决定区(RRDR)内者占96.5%(139/144),RRDR内和RRDR外同时存在突变者占99.3%(139/140);突变热点依次为rpoB531、rpoB526和rpoB516.发现未被记录的15个突变位点和4个突变类型,其中rpoB1284位点突变可能是多态性位点,与利福平耐药无关.结论:检测河南地区结核分枝杆菌rpoB基因RRDR内位点的突变可预测MDR结核分枝杆菌的耐药性.