乳癌组织中 Pax1的表达及其对乳癌 MDA-MB-231细胞生物学行为的影响

摘要

目的：研究Pax1在乳癌组织中的表达及其对乳癌MDA-MB-231细胞生物学行为的影响。方法：采用Western blot检测200乳腺原发癌和癌旁组织中Pax1蛋白的表达。采用化学合成针对Pax1基因的两个小干扰RNA （ siRNA-Pax1-1和siRNA-Pax1-2）下调该基因的表达，应用MTT和Transwell实验检测其对MDA-MB-231细胞生物学行为的影响。结果：Pax1在乳腺原发癌组织中的表达低于相应的癌旁组织，沉默Pax1表达可促进乳癌细胞增殖、迁移和侵袭（ F＝52．413和72．741，P＜0．001）。结论：Pax1有望成为乳癌早期诊断和预测预后的生物分子标志物。%To investigate the expression of paired boxed gene 1 ( Pax1) gene in breast cancer tissue and to ex-plore the influence on proliferation status , migration ability and metastatic ability of breast cancer cells MDA-MB-231 . Methods:Western blot were used to examine the expression of Pax 1 in samps, including 200 normal tissues and 200 pri-mary tumors.MTT and transwell were used to analyze the ability of proliferation , migration, and invasion in Pax1 siRNA-transfected MDA-MB-231 cells.Results: The expression of Pax1 was lower in breast cancer tissues than other tissues . Furthermore, knockdown of Pax1 expression resulted in increased proliferation and invasion in MDA-MB-231 cells in vitro (F=52.413 and 72.741,P<0.001).Conclusion:The expression of Pax1 gene shows and obvious downgraded in breast cancer tissue.Low expression of Pax1 gene can increase apeptosis and proliteration of MDA-MB-231 cells, which may pro-vide a strategy for blocking breast cancer and progression .