M1型、M2型巨噬细胞及肿瘤相关巨噬细胞中miR-146a表达的差异

摘要

目的：探讨微小RNA-146a（miR-146a）在M1型、M2型巨噬细胞及肿瘤相关巨噬细胞（TAMs）中表达的差异。方法：将单核细胞系（ THP-1）细胞诱导为M1型、M2型巨噬细胞及TAMs，应用流式细胞仪检测表型后，采用实时荧光定量PCR方法分别检测THP-1、M1型和M2型巨噬细胞及TAMs中miR-146a的表达情况。结果：THP-1细胞中miR-146a的表达量为（0．838±0．030），M1型巨噬细胞中的表达量为（1．788±0．384），M2型巨噬细胞中的表达量为（2．760±0．561），TAMs中的表达量为（2．974±0．661），差异有统计学意义（F＝12．847，P＝0．002）。其中M1型（P＝0．040）、M2型（P＝0．001）巨噬细胞及TAMs（P＝0．001）中miR-146a的表达量高于THP-1细胞， TAMs（P＝0．015）与M2型巨噬细胞（P＝0．036）中的miR-146a表达量高于M1型巨噬细胞，TAMs与M2型巨噬细胞中miR-146a的表达差异无统计学意义（P＝0．595）。结论：miR-146a在M1型、M2型巨噬细胞中的差异性表达可能与其分化和（或）细胞功能相关；TAMs的表型特征可能与M2型巨噬细胞类似，同时可能具有与M2型巨噬细胞相同的细胞功能。%Aim: To investigate the expression of miRNA-146 a in different phenotypes of activated macrophages . Methods:In this study,the THP-1 induced into M1-type,M2-type macrophages or tumor-associated macrophages(TAMs), and their phenotypes were confirmed by flow cytometry .The miRNA-146a expression was determined by qRT-PCR in M1-type macrophages ,M2-type macrophages and TAMs .Results: The miRNA-146 a expression was significantly increased in M1-type macrophages [(1.788 ±0.384), P=0.040], M2-type macrophages [(2.760 ±0.561), P=0.001]and TAMs [(2.974 ±0.661),P=0.001] compared with the THP-1 (0.838 ±0.030);The miRNA-146a in THP-1,M1-type, M2-type macrophages and TAMs had significant difference (F=12.847,P=0.002).The miR-146a expression was significant-ly increased in the M2-type macrophages(P=0.036)and the TAMs(P=0.015) compared with the M1-type macrophages. There was no statistical significance between TAMs and M 2-type macrophages in the expression of miRNA-146 a ( P =0.595).Conclusion:Different expressions of miRNA-146a in M1-type and M2-type macrophages may associate with the differentiation or their cellular functions .The phenotypic characteristics of TAMs and M 2-type macrophages are similar ,and they may have the same functions .