硫酸吲哚酚对内皮祖细胞增殖的影响

摘要

目的：观察肾毒性物质硫酸吲哚酚对人内皮祖细胞（ EPC）增殖的影响。方法：体外培养EPC，分别用0（对照）、25、50、100和200 mg／L硫酸吲哚酚刺激24、72、120 h后，MTT法测定细胞活性；分别用0（对照）、200 mg／L硫酸吲哚酚刺激24 h后，BrdU法测定细胞增殖率，流式细胞术测定活性氧（ ROS）。结果：硫酸吲哚酚体外可呈剂量和时间依赖性地抑制EPC细胞活性。200 mg／L硫酸吲哚酚作用24 h， EPC增殖受抑（P＜0．05），同时细胞内ROS大量累积（P＜0．05）。结论：硫酸吲哚酚可通过增加EPC细胞内ROS累积，抑制EPC增殖。%Aim:To investigate the effects of uremic solute indoxyl sulfate ( IS) on the proliferation of endothelial pro-genitor cells(EPC).Methods:EPC were isolated from healthy donors, then cultured by 0(the control),25,50,100 and 200 mg/L IS for 24,72,and 120 h, and the viability was measured by MTT method .EPC were cultured by 0(the control) and 200 mg/L IS for 24 h, then the proliferation was detected by BrdU assay , and the reactive oxygen species ( ROS) accu-mulation was examined by flow cytometry .Results: The viability of EPC was dose-and time-dependently inhibited by IS (P<0.05).The proliferation of EPC cultured by 200 mg/L IS for 24 h was inhibited with more ROS accumulation (P<0.05).Conclusion:IS could inhibit the proliferation of EPC via ROS .