宫颈癌组织中 FOXP3、PD-1及 PD-L1蛋白的表达

摘要

目的：研究宫颈癌组织中FOXP3、PD-1及PD-L1蛋白的表达情况及其与临床病理特征的关系。方法：采用免疫组织化学SP法检测64例宫颈癌组织、40例宫颈上皮内瘤样病变（ CIN ）组织、18例正常宫颈组织中PD-1、PD-L1、FOXP3蛋白的表达。结果：PD-1、FOXP3蛋白主要表达于肿瘤浸润淋巴细胞（ TIL），PD-L1蛋白主要表达于宫颈癌细胞及TIL。正常宫颈组织不表达PD-1、PD-L1、FOXP3蛋白，宫颈癌组织中PD-1的表达显著高于CIN和正常宫颈组织（P＜0．05）；宫颈癌组织中PD-1、PD-L1、FOXP3蛋白的表达与组织分化程度、FIGO分期有关（P＜0．05），与患者年龄、肿瘤直径、HPV感染或分型无关（P＞0．05）。宫颈癌组织中PD-L1与PD-1蛋白的表达呈正关联（rS ＝0．562，P＜0．001）。宫颈癌组织中FOXP3与PD-1、PD-L1蛋白的表达无关联（rS ＝0．218和0．257，P＞0．05）。结论：人子宫颈癌组织高表达PD-1、PD-L1、FOXP3蛋白，且与肿瘤的发生发展、浸润密切相关。%Aim:To explore the expression of PD-1, PD-L1 and FOXP3 proteins in human normal cervical tissues , cervical intraepithelial neoplasia , cervical cancer , and their relationship with clinical features .Methods: Immuno-histo-chemical SP method was used to detect the expressions of PD-1, PD-L1, FOXP3 in tissue obtained from 64 cases of cervi-cal cancer, 40 cases of cervical intraepithelial neoplasia (CIN),18 cases of normal cervical tissue.Results:FOXP3, PD-1 was mainly expressed on TIL , PD-L1was mainly expressed in cervical cancer cells and TIL .There were no expression of PD-1, PD-L1,FOXP3 in normal cervical tissues .The positive expression rate of PD-1, PD-L1, FOXP3 in cervical cancer tissues was significantly higher than that in cervical CIN tissues , and the difference was statistically significant (P<0.05);The expression of PD-1, PD-L1 and FOXP3 in cervical carcinoma was related to the degree of differentiation ,FIGO stage, the difference being statistically significant (P<0.05), and without any correlation with age , tumor size, HPV infection (P>0.05).The expression of PD-L1 and PD-1 in cervical carcinoma cells was positively correlated (rS =0.562,P<0.001).The expression of FOXP3 and PD-1 ,PD-L1 in cervical cancer tissue had no significant correlation (rS =0.218, 0.257,P>0.05).Conclusion:The expression of PD-L1 and PD-1 in human cervical cancer tissues is increased , which is closely related to the occurrence , development , infiltration and metastasis of the tumor .