浙东白鹅骨骼肌发育相关因子MRFs,Pax3和Pax7基因的表达规律

摘要

The family of myogenic regulatory factors (MRFs) played an important role in the process of skeletal muscle differentiation during the embryonic stage and the early stage of hatching with Pax3 and Pax7, two transcription factors of the PAX family, guiding the formation of various processes during the growth and devel-opment of muscle fiber. To determine the expression profiles of these genes during the embryonic and muscle development stages in geese, the expression pattern of MRFs, Pax3, and Pax7 during the embryonic period (7th, 11th, 15th, 19th, 23th, 27th day in embryonic (E) age) and early birth (7-day-age) in Zhedong White Geese was studied. Analysis included extracting RNA and synthetic DNA along with real-time PCR followed by further analysis on their relationship with the development of breast and leg muscles. We took three samples for tissue of each period, and the significance of expression was analyzed with SASS. Results revealed that MRFs, Pax3, and Pax7, were all related to the development of skeletal muscles in geese with different expression pat-terns. MRFs and Pax7 showed peak expression during the period from E11 to E15, and the highest expression of Pax3 with a declining expression appeared at E7. Findings suggested that the expression patterns of these genes were highly matched with their function during muscle development and provided a theoretical basis for further investigation of the regulatory mechanism of MRFs, Pax3, and Pax7 during skeletal muscle development in geese.%生肌调节因子家族(myogenic regulatory factors,MRFs)在胚胎期和出壳早期的骨骼肌分化过程中发挥重要作用;PAX家族中的2个转录因子Pax3和Pax7,被认为在肌纤维的生长发育过程中,指导众多过程的形成.在鹅胚胎发育过程中这些基因的表达变化规律及在肌肉发育过程中的表达变化规律研究甚少.以浙东白鹅Anser cygnoides domestica'Zhedong'为研究对象,提取RNA合成cDNA并采用实时荧光定量聚合酶链式反应(qRT-PCR)研究MRFs,Pax3和Pax7在鹅胚胎期(7,11,15,19,23,27 d胚龄)及出生早期(出雏后7日龄)的表达规律,并分析其表达与胸、 腿肌发育的相关性.结果显示:MRFs,Pax3和Pax7基因均和鹅骨骼肌的发育相关,并且存在不同的表达规律.MRFs和Pax7基因在11～15 d胚龄期间出现表达高峰期,而Pax3基因在7 d胚龄即出现高表达,随后表达下降.通过分析表明:这些基因的表达规律均和它们在肌肉发育过程中的作用密切吻合.