铁皮石斛多糖提取工艺优化研究

摘要

Polysaccharide is the most important functional ingredient in Dendrobium off icinale Kimura et Migo ,and it is important to extract the polysaccharides efficiently for the utilization of D .officinale Kimura et Migo .The extraction conditions such as extraction time ,extraction temperature and the ratio of substrate to extraction solution of polysaccharide from D .officinale Kimura et Migo by water using single factor and orthogonal experiments were optimized .The extraction ratio of polysaccharide was calculated by subtract between the total polysaccharide and monosaccharide ,which were analyzed by phenol sulfuric acid method and DNS method , respectively .The single factor experiments results showed that the ratio of substrate to water of 1∶120 ,extraction temperature of 70 ℃ and extraction time of 90 min are the optimal conditions ,which are also of the optimal extraction conditions of orthogonal experiments .The identified experiments showed that under the optimal conditions the maximal extraction quantity is of 55 .2% .%多糖为铁皮石斛中的主要有效成分，高效浸出有利于其进一步利用。以水为溶剂，对影响铁皮石斛多糖提取的时间、温度及料液质量比等因素进行了优化研究，并应用苯酚‐硫酸法和DNS法考察了铁皮石斛提取液总糖和单糖的提取率，多糖提取率由两者之差计算得出。单因素实验和正交实验结果表明，料液质量比1∶120、浸提温度70℃、浸提时间90 min时提取铁皮石斛多糖效果最好，在此工艺条件下，铁皮石斛多糖提取率为55．2％。