非离子表面活性剂对丙酮丁醇梭菌发酵的影响

摘要

With the enhancement of increasing environmental protection consciousness and depletion of fossil fuels ,biomass energy is being developed .In order to solve the problem of the high cost of fermentation substrate and low butanol productiticy influencing biobutanol fermentation ,the accelerator in fermentation of acetone‐butanol‐ethanol (ABE ) solvent by Clostridium acetobutylicum was investigated . The enzymatic hydrolysate of lignocellulosic biomass was used as the carbon substrate for C . acetobutylicum fermentation ,the nonionic surfactants such as Tween‐80 and polyethylene glycol 6000 (PEG 6000 ) was used as fermentation accelerator .The results showed that Tween‐80 and PEG 6000 can significantly improve the yields of ABE ,but reducing maximum dry cell weight and the specific grow th rate of C . acetobutylicum . This provides basic parameters for improving the fermentation of biobutanol , and establishes a basis for biobutanol fermentation using hydrolysates of lignocellulose as substrate .%环境保护意识的增强和化石燃料的日益枯竭，促进了生物质能源的开发。为了解决生物丁醇燃料工业化发酵底物成本高而丁醇产量较低的问题，今探讨影响丙酮丁醇梭菌发酵产丙酮‐丁醇‐乙醇（ABE ）溶剂的促进剂。以生物质木质纤维素的水解产物作为丙酮丁醇梭菌的发酵底物，利用非离子型表面活性剂 T w een‐80和聚乙二醇（PEG ）6000作为丙酮丁醇梭菌的发酵促进剂。结果表明，添加 T w een‐80和PEG6000，降低了丙酮丁醇梭菌的比生长速率和菌体最大质量浓度，提高了发酵产物ABE的产量，从而为发酵法提高生物丁醇产量提供基础参数，也为利用木质纤维素的水解液发酵生产生物丁醇提供了基本依据。