丙酮丁醇梭菌复合诱变及发酵废弃物原料产生物丁醇的研究

摘要

[Objective] The aim was to study better fermentation medium for new type biofuel butanol through recycling waster resources.[Method] Clostridium acetobutylicum UM-80,a mutant with high butanol yield and good stability,was obtained by ultraviolet mutagenesis and co-mutagenesis with Fe2+ by Clostridium acetobutylicum CGMCC 1.0134.Then the different fermentation materials were used to study the fermentation performance of the mutant strains,and the suitable medium with high cost performance was found out.[Result] The yield of butanol and total solvent (acetone,butanol,ethanol) were 9.04 and 17.95 g/L,respectively,which were 5.12％ and 4.12％ higher than those of the original strain.The fern root was the best raw material for the fermentation of butanol;when the mass fraction of fern root was 15％,the concentration of butanol in fermentation broth was 5.50 g/L.When the co-fermentation of Mortierella alpina and fern root,the highest yield was 6.50 g/L.[Conclusion] The co-fermentation of Mortierella alpina and fem root is better fermentation medium for butanol.%[目的]通过废弃资源的再利用研究可替代的新型生物燃料——丁醇的发酵培养基.[方法]对菌株Clostridium acetobutylicuCGMCC 1.0134进行紫外诱变和磁场与Fe2共同诱变,获得1株丁醇产量高和稳定性好的优良突变株Clostridium acetobutylicum UM-80,采用不同的废弃原料考察该突变菌株的发酵性能,并筛选出合适的性价比高的培养基.[结果]突变株UM-80发酵产丁醇和总溶剂(丙酮、丁醇、乙醇)分别为9.04、17.95 g/L,较原始菌株分别提高了5.12％、4.12％.单独的蕨根是较好的丁醇发酵原料,当蕨根醪质量分数为15％时发酵液中丁醇浓度最高为5.50 g/L.当高山被孢霉与蕨根共同发酵时发酵液中丁醇最高产量为6.50 g/L.[结论]蕨根与高山被孢霉的复合培养基是较好的生物丁醇发酵培养基.