一株高产灵菌红素粘质沙雷氏菌的筛选与鉴定

摘要

A red pigment producing bacterial strain was screened from a freshwater fish body through isolating color bacteria in nature. This strain was identified as Serratia marcescens based on morphological, physiological characters and 16S rDNA gene sequence. This pigment was demonstrated belong to prodigiosin on the basis of UV spectrum and TLC. The yield of crude prodigiosin reached at 475 mg/L.%通过筛选自然生境中的产色素菌株,从淡水鱼体中分离得到一株高产红色色素的Sm-128菌株.经形态观察、生理生化实验和16S rDNA基因序列分析,鉴定Sm-128菌株为粘质沙雷氏菌（Serratia marcescens）.结合紫外吸收光谱及薄层色谱分析,证实Sm-128菌株所产色素为灵菌红素,粗品产量达475 mg/L.