老年慢性支气管炎患者肺炎嗜衣原体感染的检测诊断150例分析

摘要

[ObjectiveJTo explore the detection method of chlamydophila pneumonia infection and its clinical significance. [Methods]75 cases of elderly patients with chronic bronchitis as the experimental group and 75 cases of elderly healthy patients as the control group. The CPN-IgG antibody and CPN MOMP amplification of the gene were detected by ELISA and PCR assay, respectively. [Results]The positive rates of CPN MOMP and CPN-IgG were 64.0% and 89.3%, respectively, significantly higher than the control group with the values of 12.0% and 25.3% . [Conclusion] ELISA and PCR methods for detection of CPN, can improve the diagnostic accuracy of the old chronic bronchitis.%[目的]探讨老年慢性支气管炎(chronic bronchitis,CB)患者血清中肺炎嗜衣原体(chlamydophila pneumonia,CPN)感染的检测方法及其临床意义.[方法]75例老年CB患者为实验组,75例健康老年志愿者为对照组；分别采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)和聚合酶链反应(polymerase chain reaction,PCR)法检测研究对象的肺炎嗜衣原体IgG(chlamydophila pneumonia-IgG,CPN-IgG)和主要外膜蛋白(major outer membmne protein,MOMP):CPN MOMP也称为外膜蛋白A(存在于Cpn原体和网状体,主要包括MOMP).实验组与对照组均用了上述两种方法.[结果]实验组CPN MOMP阳性、CPN-IgG阳性的构成比分别为64.0％和89.3％,均明显高于对照组的12.0％、25.3％,差异均具有统计学意义(P＜0.05).结果显示,PCR和ELISA两种检测方法均能反映发生肺炎嗜衣原体感染.[结论]ELISA和PCR法均可对CPN进行检测,两种方法同时运用能提高老年CB的临床诊断率,降低假阴性的发生,利于改善预后.