基于HOTAIR高表达的U87细胞的恶性增殖以及与干扰素的相关性研究

摘要

Ojective] Researching the relationship of interferon, HOTAIR(HOX gene transcript antisense RNA) and malignant glioma to explore molecular mechanism of tumorigenesis and development. [Methods] Analyzing the differences of expression of HOTAIR in tumor tissue and normal tissue by GSE4290 datasets which is downloaded from gene expression database from NCBI website and analyzing the differences of expression of HOTAIR in glioma cells and normal glial cells by RT-PCR results. Testing the U87 glioma cell that treated by siHOTAIR transfection proliferation and cycle by MTT method and flow cytometry. Detecting the HOTAIR gene expression of U87 glioma cells stimulated by interferon alpha, beta or gamma via RT-PCR. [Results] The expression of HOTAIR in U87 cell is significantly higher than in normal tissue, inhibiting HOTAIR expression could reduce the proliferation rate of glioma cell and increase the cell cycle arrest. There was no significant differencein HOTAIR expression of U87 cells stimulated by interferon beta or gamma( P>0.05); but there was significant differenceinthe expression of HOTAIR in U87 cells stimulated by interferon alpha increased and this effect was further enhanced at the longer time and higher concentration(P<0.05, P<0.01).[Conclusion] Altered HOTAIR expression may promote glioma development and interferons alpha might participate in the development of glioma though increasing the HOTAIR expression.%[目的]研究干扰素(interferon,IFN)、HOX基因反义转录RNA (HOX gene transcript antisense RNA，HOTAIR)和恶性胶质瘤的关系，探索恶性胶质瘤发生发展的分子机制。[方法]从NCBI网站的基因表达数据库下载GSE4290数据集分析HOTAIR在肿瘤组织和正常组织中的表达差异；使用qRT-PCR分析HOTAIR在人脑胶质瘤细胞U87和人星形胶质细胞HA1800中表达差异；使用MTT法和流式细胞术检测siHOTAIR转染对U87细胞增殖和周期的影响；使用qRT-PCR检测干扰素α、β和γ干预U87细胞后HOTAIR基因的表达情况。[结果]肿瘤组织中HOTAIR表达量明显高于正常组织，U87细胞中的HOTAIR表达量显著高于HA1800，抑制HOTAIR高表达可降低U87细胞的增殖率并且可增加细胞周期的阻滞，IFN-β和IFN-γ干预U87细胞后HOTAIR的表达量差异无统计学意义（P>0.05）；IFN-α干预U87细胞后HOTAIR的表达量增加，干预时间越长、浓度越高表达量增加越显著，差异有统计学意义（P<0.05，P<0.01）。[结论]HOTAIR在U87细胞中异常表达可能对胶质瘤发生发展具有促进作用，IFN-α能够上调HOTAIR的表达，参与胶质瘤的发生发展。