Uniform design was used to optimize semi-bionic extraction( SBE)process of Lotus leaf. The optimum process conditions of semi-bionic extraction of Lotus leaf were studied with total flavonoids and dried extract weight as the index. Be-sides,effects of the pH value and extraction time of the three-time extraction were investigated. Spectrophotography method was conducted to determine the content of total flavonoids. Combined with the industrial production practice,the optimal ex-traction process was as follows:pH value of water in the three-time extraction was 3. 5,6. 5 and 9. 0,with the extraction time of 2. 0 h,1. 5 h and 1. 5h,respectively. The obtained method was easy and simple to operate and the extract rate of total fla-vonoids was relatively high,which provided a theoretical basis for the extraction optimization of Lotus leaf.%采用均匀设计法优选荷叶半仿生提取的最佳工艺条件.本研究以荷叶主要有效成分总黄酮得率以及干浸膏得率为评价指标，对荷叶的半仿生提取工艺条件进行优选，考察三煎pH、提取总时间对荷叶总黄酮提取率的影响，采用分光光度法测定总黄酮的含量.通过优化实验并结合生产实际，确定荷叶半仿生提取的最佳工艺为3煎pH依次为3.5、6.5、9.0，3煎时间依次为2.0 h、1.5 h、1.5 h，所得荷叶总黄酮的提取率为9.7﹪.本法操作简便，提取率较高，为荷叶总黄酮提取工艺的改进提供了理论依据.
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