棉酚对多浪羊淋巴细胞凋亡及其对核酸降解的研究

摘要

In this study, the effects of gossypol on Duolang sheep lymphocyte apoptosis and degradation of nucleic acid on were studied by culturing lymphocytes in RPMI 1640 medium, Wright-Giemsa staining, DNA and RNA gel electrophoresis. The results showed that; lymphocyte appeared apoptotic bodies after Wright-Giemsa stained for 4 h; DNA and RNA were detected by Agarose Gel Electrophoresis. The DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis. The RNA agarose gel electrophoresis appeared 18S rRNA began to degrade at 4th hours. It was concluded that after treated by 1. 35μmol/L of gossypol for 4 h, the lymphocytes could inhibit lymphocyte proliferation of Duolang sheep , and promote apoptosis and degradation of nucleic acid.%主要通过使用RPMI 1640培养基培养淋巴细胞、瑞氏一吉姆萨染色、DNA和RNA电泳等方法来研究棉酚对多浪羊淋巴细胞的凋亡效应和对淋巴细胞核酸影响.研究结果表明,经瑞氏一吉姆萨染色4h淋巴细胞出现凋亡小体；DNA和RNA通过琼脂糖凝胶电泳检测在4h分别出现梯状条带和18S降解.可见,当细胞培养液中棉酚浓度为1.35 umol/L,培养淋巴细胞4h后,可抑制多浪羊淋巴细胞的增殖,并且促进凋亡及其核酸的降解.