Objective To discusses the effects of valdecoxib on the migration and invasion of human colorectal cancer Colo320 cells and possible mechanisms involved .Methods Colo320 cells were exposed to different concentra-tions of valdecoxib (0, 50, 100, and 200 mol/L) for 24 h.Then, the effects of valdecoxib on the migration and inva-sion/metastasis of Colo320 cells were determined by wound healing assay and Transwell method .The levels of COX-2, MMP-2 and E-cadherin in Colo320 cells were measured by Western blotting .Results Exposure to 50, 100 and 200 mol/L of valdecoxib could obviously inhibit the migration of Colo 320 cells, compared with the control (F=399.1, P<0.01).According to Transwell assay results , the number of invasive cells were remarkably lower in the valdecoxib treat-ment group than that in the control (F=36.5, P<0.01).Compared with the control, the valdecoxib treatment group produced markedly decreased levels of COX -2 (F=52.04, P<0.01) and MMP-2 (F=100.3, P<0.01), but in-creased amounts of E -cadherin (F=60.3, P<0.01).Conclusion Valdecoxib can inhibit the migration and invasion of Colo320 cells, which may be associated with inhibition of COX -2 and MMP-2 expression and up-regulation of E-cadherin protein .%目的：研究非甾体抗炎药戊地昔布（valdecoxib）对人直肠癌Colo320细胞迁移及侵袭能力的影响，并初步探讨其可能的机制。方法用不同浓度（0、50、100、200μmol／L）的戊地昔布分别处理Colo320细胞24 h，采用划痕及Transwell小室实验检测细胞迁移及侵袭能力的变化；采用Western blot实验检测戊地昔布作用于Colo320细胞后细胞内环氧化酶－2（COX－2）蛋白的表达变化，并检测与迁移、侵袭相关的基质金属蛋白酶－2（MMP－2）及钙黏附蛋白－E（E－ceherin）的表达变化。结果不同浓度的戊地昔布分别作用于Colo320细胞24 h后，50、100及200μmol／L戊地昔布处理组细胞的迁移率较对照组明显下降，与对照组相比差异具有统计学意义（ F＝399．1，P＜0．01）；Transwell实验结果显示戊地昔布处理组侵袭细胞数较对照组明显减少，与对照组比较差异具有统计学意义（F＝36．5，P＜0．01）；Western blot实验结果显示戊地昔布处理组相对于对照组细胞内COX－2蛋白表达量明显降低（F＝52．04，P＜0．01），E－ceherin表达量增加（F＝60．3，P＜0．01），MMP－2蛋白表达量减少（F＝100．3，P＜0．01）。结论戊地昔布可以抑制直肠癌Colo320细胞的迁移及侵袭能力，其机制可能是通过抑制COX－2的表达实现的。此外，还可能是通过调节与EMT相关的MMP－2及E－caherin蛋白的表达而实现的。
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