枳壳药材水提液 HPLC 指纹图谱研究

摘要

Objective To establish HPLC fingerprints of water extract fromCitrus aurantium L.Methods The sample solution was prepared by refluence and HPLC was employed to separate ingredients in Citrus aurantium L by gradient elution with mixture of acetonitrile and 0.1% formic acid and detected at 254 nm. The fingerprint data were analyzed by similarity evaluation.Results 12 common peaks were defined in dif-ferent fingerprints and 3 characteristic peaks were identified as naringin,isonaringin,and neohesperidin. The relative standard deviation (RSD)values of the relative retention time of 12 common peaks were lower than 0.805% when peak 5 (neohesperidin)was taken as reference peak.It was indicated that the present method was stable and reproducible.But,the RSD values of the relative peak area varied with fluctuation (-196.7%),which indicated that the content of active compounds in C.aurantium changed largely.Con-clusion This is the first HPLC fingerprint investigation on water - solubility ingredients for Citrus aurantium L and it could supply an effective guide for clinical drug use and collecting periods of Citrus aurantium L with water as extracting solvent.%目的：建立枳壳水提液中黄酮类成分特征指纹图谱方法。方法采用回流方法制备枳壳供试品溶液，以乙腈-0．1％甲酸水溶液梯度洗脱，检测波长254 nm，建立枳壳水提液的 HPLC 指纹图谱，对在江西省不同时间采收的枳壳药材进行相似度评价分析。结果从15批不同供试品指纹图谱中确定了12个共有峰，通过与对照品比对，指认了其中的3个特征峰，分别为柚皮苷、新橙皮苷、异柚皮苷。以新橙皮苷峰为参照峰，15批样品指纹图谱中12个共有峰的相对保留时间的相对标准偏差（RSD）均＜0．805％。12个共有峰的相对峰面积变化较大， RSD 值为196．7％。结论该方法具有较好的重现性和稳定性，不同样品中化学成分的含量变化较大，可对枳壳临床用药及采收期提供指导。