PHARMACOGNOSTIC STUDIES AND HPTLC FINGERPRINTING OF BLUMEA ERIANTHA DC (ASTERACEAE) LEAVES

摘要

Objective: To study the pharamacognostic, morphological, microscopical characters of leaves of Blumea eriantha DC also to carry out the chemical analysis of its leaf powder and establish the chromatographic fingerprint. Methods: Anatomical investigations and fluorescence analysis were carried out as per the standard techniques. Various quantitative parameters like ash values, extractive values and moisture content can be used as quality control parameters for B. eriantha were determined. The air dried leaf powder was extracted with methanol and fingerprinting pattern was developed by using High Performance Thin Layer Chromatography (HPTLC) technique. Results: The epidermal study of leaves revealed several stalked glandular trichomes and multicellular uniseriate trichomes on both the epidermis of the leaves. Fluorescence analysis of leaves powder of B. eriantha revealed a range of colours from dark green to brownish black under short Ultra Violet (UV) light. The HPTLC fingerprint of methanolic extract of B. eriantha leaves showed six well resolved components. Conclusion: The pharamacognostic studies carried out for Blumea eriantha DC and presented here provide referential information for the identification of this crude drug and will also help in distinguishing it from its adulterants. Keywords : Blumea eriantha , Phamacognostic studies, Fluorescence analysis, HPTLC, Trichome.