黑木耳提取物对感染性休克大鼠肝、肠组织MDA 及 GSH-Px 的影响

摘要

目的：探讨黑木耳提取物对感染性休克大鼠肝、肠组织丙二醛（MDA）及谷胱甘肽过氧化物酶（GSH‐Px）的影响。方法将50只健康Wister大鼠随机分为对照组（假手术组）、模型组（感染性休克组）、阳性对照组（思密达组）、黑木耳粗提物组、黑木耳多糖组5组，每组10只。各组大鼠连续灌胃3d，每d3次，分别灌注生理盐水、生理盐水、思密达、黑木耳粗提物、黑木耳多糖，灌注量为1．5 m L／100 g体重。在乙醚麻醉下行盲肠结扎穿刺术，6 h后取大鼠腹主动脉血液及时检测血气，取肝、肠及肺组织光镜下观察形态学变化，并检测肝、肠组织MDA及GSH‐Px的含量。结果与对照组比较，模型组大鼠肝、肠内 MDA 浓度升高，黑木耳粗提物组大鼠肝脏内MDA浓度升高，差异有统计学意义（P ＜0．05）；与模型组比较，思密达组和黑木耳多糖组大鼠肝、肠组织内MDA浓度降低，黑木耳粗提物组大鼠小肠内MDA浓度降低，差异有统计学意义（P ＜0．05）。与对照组比较，模型组和黑木耳粗提物组大鼠小肠内GS H‐Px水平下降，差异有统计学意义（ P ＜0．05）；与模型组比较，思密达组和黑木耳多糖组大鼠小肠内GS H‐Px水平升高，差异有统计学意义（ P ＜0．05）；与对照组比较，其余4组大鼠肝脏内GS H‐Px浓度均降低，差异有统计学意义（ P ＜0．05）；与模型组比较，黑木耳粗提物组和黑木耳多糖组大鼠肝脏内GS H‐Px浓度升高，差异有统计学意义（P ＜0．05）；与思密达组比较，黑木耳多糖组GSH‐Px浓度升高，差异有统计学意义（P ＜0．05）。光镜观察：对照组肝脏及小肠组织正常，模型组肝细胞呈片状水肿，小肠黏膜上皮细胞破坏，肺间质水肿，其余3组组织上述表现则较轻，基本正常。结论黑木耳多糖能有效控制感染性休克时小肠和肝脏内有害物质MDA上升的水平，减缓GSH‐Px下降的水平，且其对肝脏内GSH‐Px的影响优于思密达。%Objective To study the effects of extract from black fungus on liver and small intestine′s malon‐dialdehyde (MDA) ,glutathione peroxidase (GSH‐Px) in the septic shock rat ,as well as the protection of the liver and small intestine′s function during the failture caused by septic shock .Methods Fifty healthy Wistar rats were randomly divided into 5 groups:the blank control (the sham‐operation) group ,the tes‐ting (the septic) group ,the smectite(the montmorillonite power) group ,the black fungus crude extract group and the black fungus polysaccharids group .Respectively undertook saline ,saline ,smectite ,black fungus crude extract ,black fungus polysaccharids to each group on three consecutive days .And the perfu‐sion was 1 .5 mL/100 g ,three times a day .Then it was filled again 180 min before establishing the mod‐els .Established the models by performing ligation and perforation on cecum under the ether .After 6h , draw off blood of the abdominal aortic to analyze blood‐gas ,and observed the form on liver ,small intestine and lung ,as well as measured the content of MDA ,GXH‐Px in liver and small intestine .Results The concentration of MDA in liver ,small intestine of the testing group and the concentration in liver of the black fungus crude extract group were higher than it of control group ,the differences were statistical sig‐nificant (P <0 .05);MDA in liver ,small intestine of the smectite group ,the black fungus polysaccharids group and in small intestine of black fungus crude extract group were lower than it of testing group ,the differences were statistical significant (P <0 .05) .The concentration of GSH‐Px in the testing group ,the black fungus crude extract group were lower than it of control group ,the differences were statistical sig‐nificant (P <0 .05);The concentration of the smectite group ,the black fungus polysaccharids group were higher than it of testing group ,the differences were statistical significant (P <0 .05);The concentration of GSH‐Px in the last four groups were lower than it of control group ,the differences were statistical sig‐nificant (P <0 .05) .The concentration of GSH‐Px in the black fungus crude extract group and the black fungus polysaccharids group were higher than it of testing group ,the differences were statistical significant (P <0 .05) .The concentration of GSH‐Px in the black fungus polysaccharids group were higher than it of smectite group ,the differences were statistical significant (P <0 .05) .Observed under the light micro‐scope:The liver ,small intestine of the control group were normal .In the testing group ,the cell of liver were edema ,the epithelial cells of mucosa were damaged .But the above‐mentioned performances in the other three groups were less ,practically approaching normal .Conclusion The black fungus polysacchar‐ids can control the harmful substance‐MDA to rise and slow down the decreasing of the GSH‐PX in the septic shock ,as well as it was better than smectite on GSH‐Px of liver .