lncRNA H19在食管鳞癌细胞KYSE30中的作用与功能

摘要

目的 研究长链非编码 RNA(long non-coding RNA,lncRNA)H19在食管鳞癌细胞中的作用与功能.方法 运用实时荧光定量PCR(Real time quantitative polymerase chain reaction,qRT-PCR)检测5种食管鳞癌细胞系中 lncRNA H19本底表达,用RNA干扰技术转染 H19小干扰RNA(Small interfering RNA,siRNA)至食管鳞癌细胞KYSE30;实验分3组:空白对照组(空白载体组)、阴性对照组(随机序列组)和实验组(H19干扰组).用噻唑蓝比色法(MTT)、流式细胞术(Flow Cytometry,FCM)、细胞划痕、Transwell侵袭实验检测 lncRNA H19对食管鳞癌细胞增殖、凋亡、迁移及侵袭能力的影响.结果 5种食管鳞癌细胞系中,KYSE30细胞 IncRNA H19表达量最高,其次分别是 Eca109、KYSE150、EC9706和 KYSE450细胞.实验组敲低 KYSE30细胞中 lncRNA H19的表达(P <0.05),细胞增殖受到抑制(P <0.05),细胞凋亡增加(P <0.05),细胞迁移距离明显减小(P <0.05),细胞侵袭能力明显减弱(P <0.05),与空白对照组和阴性对照组相比,差异有统计学意义.结论 lncRNA H19的表达能够促进食管鳞癌细胞的增殖、迁移和侵袭,并抑制细胞凋亡,食管癌中 lncRNA H19发挥促进细胞恶性增殖及浸润、转移的癌基因作用,实验结果为今后可能用于临床诊断及治疗奠定了基础.%Objective To investigate the role of lncRNA H19 in esophageal squamous cell carcinoma(ES-CC).Methods The expressions of lncRNA H19 in 5 ESCC cell lines were detected by qRT-PCR.H19 siR-NA was transfected into KYSE30 cell to knockdown the expression of lncRNA H19,and the study was di-vided into the normal control,nagetive control and experimental groups.The effects of H19 on cell prolif-eration,apoptosis,migration and invasion were investigated by MTT,FCM,Wound-healing and Tran-swell invasive assay.Results The expression of lncRNA H19 was high in 5 ESCC cells.The knockdown expression of H19 in KYSE30 cellssuppressed cell proliferation(P <0.05),migration(P <0.05)and in-vasion(P<0.05),meanwhile accelerated cell apoptosis(P<0.05).Conclusion lncRNA H19 plays a role of oncogene in ESCC,thus probably promotes ESCC malignant proliferation and distant metastasis.