IL-1β引起食管鳞状细胞癌细胞上皮-间质转化的实验研究

摘要

Objective To investigate the effect of IL-1β on epithelial mesenchymal transition (EMT) in ESCC cells.Methods IL-1β was added to the cell culture medium,and the morphological changes of ESCC cells and EMT related markers were observed 48 h after IL-1β treatment.Cells added PBS was the untreated group.Results The morphology of ESCC cells was altered dramatically from a typical epithelial-like shape of mesenchymal,qRT-PCR and Western blot revealed that IL-1β treatment resulted in down-regulated the epithelial marker E-cadherin (P＜0.05) and up-regulated the mesenchymal marker Vimentin and Slug (P＜0.05).Conclusion IL-1β is able to induce EMT in ESCC cells.%目的 研究白细胞介素1-β(IL-1β)对食管鳞状细胞癌(ESCC)细胞上皮-间质转化(EMT)的影响.方法 在细胞培养基中加入IL-1β,观察IL-1β处理48 h后ESCC细胞形态及EMT相关标志物的变化,另以加入PBS的ESCC细胞为阴性对照组.结果 IL-1β处理后ESCC细胞由上皮样形态转变为间质样形态.qRT-PCR与Western blot结果显示,IL-1β处理后上皮标志物E-钙黏蛋白(E-cadherin)表达减少,间质标志物波形蛋白(Vimentin)和Slug表达增加,与对照组比较差异有统计学意义(P＜0.05).结论 IL-1β可引起ESCC细胞发生EMT.