Objective To identify the differences of epithelial mesenchymal transition (EM T ) re-lated biomarkers of E-cadherin and Vimentin in mRNA and protein expression levels between esophageal squamous cell carcinoma (ESCC) with lymph node metastasis and no lymph node metastasis . Methods Immunohistochemistry SP method and quantitative reverse transcriptase real-time polymerase chain reac-tion (qRT-PCR) array were applied to detect mRNA and protein expression levels of E-cadherin and Vim-entin on 47 cases of ESCC with lymph node metastasis and 30 cases of ESCC without lymph node metasta-sis ,and 20 cases of normal esophageal mucous membrane tissues . Results The percentage of expressing E-cadherin in normal control group ,non metastasis and metastasis group of ESCC were 20/20 (100% ) , 29/30 (96 .67% ) ,and 44/47 (93 .62% ) respectively ,there was a statistically significant difference among three groups ( P<0 .05) ,but the percentage of high E-cadherin expression of metastasis group (22/47 , 46 .8% ) and non metastasis group (18/30 ,60% ) had no significant difference (P>0 .05) . The number of cases with the positive Vimentin protein expression increased in order of normal control group ,no me-tastasis group and metastasis group ,the percentage was (0/20 ,0) ,(11/30 ,36 .67% ) ,(37/47 ,78 .72% ) respectively ,showing a significant difference between two groups (P<0 .05) . The rate with high ex-pression level of Vimentin between the two groups of metastasis group (11/47 ,23 .4% ) and no metastasis (2/19 ,10 .53% ) had statistical significance (P<0 .05) . There was a significant negative correlation be-tween Vimentin and E-cadherin ,the correlation coefficient was r= -0 .377 . qRT-PCR analysis showed that there was a obvious difference in mRNA expression levels of E-cadherin and Vimentin in the normal group ,no metastasis group and metastasis group of ESCC (P< 0 .05) . E-cadherin mRNA expression level showed no significant difference between metastasis group (0 .43 ± 0 .23) and non metastasis group (0 .51 ± 0 .54)(P> 0 .05) ,while that of Vimentin had a significant difference between metastasis group (4 .05 ± 1 .88) and non metastasis group (2 .70 ± 1 .73) (P< 0 .05) . Conclusions The expressions of EM T related genes E-cadherin and Vimentin were closely correlated with metastasis of ESCC ,and there-fore could be used for predictors of the metastasis and recurrence of ESCC as well as useful biomarkers to prognosis .%目的：观察上皮间质转化相关标记物E-钙黏蛋白（E-cadherin）和Vimentin基因及其蛋白在食管鳞状细胞癌淋巴结转移组和非转移组中的表达，并分析E-cadherin和Vimentin表达的相关性。方法采用免疫组织化学SP法和实时定量逆转录聚合酶链反应（qRT-PCR）检测47例有淋巴结转移、30例无淋巴结转移的食管癌和20例正常食管组织中E-cadherin和Vimentin基因及其蛋白的表达情况。结果对照组、非转移组与转移组 E-cadherin蛋白表达分别为20/20（100％）、29/30（96．67％）和44/47（93．62％），组间比较差别有统计学意义（ P＜0．05）。转移组的高表达率22/47（46．8％）低于非转移组18/30（60％），但差别无统计学意义（ P＞0．05）。Vimentin蛋白在对照组、非转移组、转移组阳性表达率逐渐增高，分别为0/20（0）、11/30（36．67％）、37/47（78．72％），组间比较差别有统计学意义（ P＜0．05），转移组与非转移组间比较差别有统计学意义（ P＜0．05）。Vimentin和E-cadherin表达呈显著负相关，相关系数（ r＝-0．377）。qRT-PCR检测显示非转移组和转移组中E-cadherin和Vimentin mRNA表达量与对照组比较差别有统计学意义（P＜0．05）。E-cadherin基因的表达在转移组（0．43±0．23）与非转移组（0．51±0．54）间的差别无统计学意义（ P＞0．05），而转移组 Vimentin表达（4．05±1．88）明显高于非转移组（2．70±1．73），差别有统计学意义（ P＜0．05）。结论上皮间质转化相关基因E-cad-herin和Vimentin与食管鳞癌的转移密切相关，可作为预测食管癌转移复发及评估临床预后的有效标记物。
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