甘蓝型油菜SRAP标记遗传多样性分析

摘要

The genetic diversity of 43 Brassica napus L.,which came from domestic and abroad,was ana-lyzed by SRAP markers.The results showed that 44 pairs of primers performed 3 1 2 allele loci,and the per-centage of polymorphism locus was 52.5% with 164 loci.The range of polymorphic loci per primer was 2-8,the average number of polymorphic loci was 3.8.The range of PIC was 0.374-0.856,the genetic similari-ty coefficient was between 0.55-0.89 with the average of 0.699.There were great differences between test-ed materials to set 0.63 as the threshold value,43 tested materials were classified into 3 large groups.The groups were relative independent,and had a centain degree of permeability,indicating the presence of ge-netic relationships between different materials.%用SRAP分子标记对国内外43份甘蓝型油菜材料进行遗传多样性分析,筛选出44对多态性好的SRAP标记.44对多态性好的引物共检测到312个等位变异位点,其中多态性位点164个,多态性比率为52．5％,每对引物约2~8个多态性位点,平均每对引物为3．8个多态性位点.多态性信息含量0．374~0．856,平均为0．699.遗传相似系数在0．55~0．89,参试材料差异较大.以0．63为阈值将43份参试材料划3大类群,3大类群间相对独立又有一定程度的渗透,说明材料之间存在不同程度的亲缘关系.