Genetic Diversity Analysis of Cotoneaster schantungensis Klotz. Using SRAP Marker

摘要

In order to detect the genetic relationship of different Cotoneaster schantungensis natural populations, 16 samples were selected for genetic diversity analysis based on SRAP markers. Twelve pairs primers were selected out, 93 bands were amplified, of which 91 bands (97.85%) were polymorphic. At species level, the average value of Nei’s genetic diversity (H) was 0.2213, Shannon’s information index (I) was 0.3596, whereas at population level, they were 0.1955, 0.3121, the percentage of polymorphic loci was 75.27%. The genetic differentiation coefficient in populations (Gst) was 0.0788, gene flow Nm was 5.8467, indicating that there was 7.88% of the variation among populations and 92.12% of the variance within populations and the variation within populations was relatively high. These results indicate that C. schantungensis has high genetic diversity and low differentiation among populations. Although most Cotoneaster species are apomictic, C. schantungensis, however, may be an exception.