一种新的鸡胚尿囊膜模型的建立与应用

摘要

Objective To construct a new model of chicken chorioallantoic membrane (CAM) and investigate the inhibition of hydrocortisone on CAM angiogenesis. Methods The CAM was constructed by air chamber. The carriers of silastic ring, methylcellulose, gelatin sponge and fiberglass filter paper were used. Results The new CAM model was ideal for anti-angiogenesis drug screening. The carrier of silastic ring had the property of direct-viewing, accurate application of sample, and convenient observation. Hydrocortisone had a good inhibitory effect on angiogenesis of CAM in the dose-effect manner. Conclusion The new CAM model is a tool for angiogenesis research. Hydrocortisone has an obvious inhibitory effect on angiogenesis of CAM by CAM model.%目的 建立一种新的鸡胚尿囊膜模型并考察氢化可的松对鸡胚尿囊膜血管生成的影响.方法 运用假气室法建立鸡胚尿囊膜模型,采用硅胶环、甲基纤维素、明胶海绵、玻璃纤维滤纸为给药载体进行考察.结果 用硅胶环作为载体建立的鸡胚尿囊膜实验模型,是一种较理想的研究抗血管生成药物的筛选模型.硅胶环作为载体与明胶海绵和纤维滤纸载体等相比,具有效果直观,加样量准确,在显微镜下易于观察、照相的优点.氢化可的松对鸡胚尿囊膜新生血管生成具有较好的抑制作用,呈现出较好的量效关系.结论 本研究建立了一种新的鸡胚尿囊膜模型,运用该模型得出氢化可的松对鸡胚尿囊膜血管生成有明显的抑制作用.