METHOD FOR CREATING A MODEL FOR STUDYING THE PHYSIOLOGY AND PATHOLOGY OF A PRENATAL PERIOD OF ONTOGENESIS ON A CHICKEN EMBRYO

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to experimental medicine, and can be used to create a model for studying the physiology and pathology of prenatal ontogenesis on a chicken embryo. Chicken embryo is cultured without a shell. 30 ml of benzyldimethyl [3- (myristoylamino) propyl] ammonium chloride monohydrate is poured onto the bottom of the polyethylene cartridge. Polyethylene film is freely placed into the cup to form a "sack" and gives it an ovoid shape. 8 holes with diameter of 7–9 mm are made along perimeter of film, in its upper part. 450–500 mg of calcium alginate and 3 ml of highly purified water are added to the bottom of the "bag" formed from the film. Then the fertilized one is transferred into the produced "bag", held during 45–50 hours at T 38 °C and humidity of 80 %, chicken egg, from which, by means of globular hard-alloy boron and micromotor with straight tip and tweezers for micromanipulations after treatment with chlorhexidine, a shell and a support shell are removed. After that, yolk region is applied with syringe 0.4 mg of gentamycin sulphate and polyethylene film is tightened from above on cup. Sleeve is placed in an incubation thermostat at temperature of 38 °C and humidity of 80 % with inclination at angle of 8–10°. Twice a day, the sleeve is inclined in the opposite direction. On 6th day in the lateral surface slightly above the liquid level, 7 mm hole is made and from 17th day of incubation through it from an oxygen concentrator an oxygen-enriched air mixture fed through a humidification purifier with an ultrasonic evaporator is supplied by means of a tube of the appropriate diameter. On 21st day a live chicken is removed from the cup.;EFFECT: method provides culturing a chicken embryo ex ovo to produce a live chicken on 21-22th day of incubation by using a special system for cultivation based on widely available components, conditions in which are close to natural.;1 cl, 3 dwg, 2 ex