变应性鼻炎患者血液嗜酸性粒细胞富集群中TLR2、TLR4、TLR7和TLR9的变化及其相关性

摘要

目的 探讨变应性鼻炎(AR)患者血液嗜酸性粒细胞富集群中 TLR2、TLR4、TLR7 和 TLR9 的变化及其相关性,探索其在 AR中的发病机制.方法 收集健康人和 AR患者的外周静脉血,用蒿草花粉、尘螨和梧桐花粉过敏原提取液刺激,流式细胞术检测刺激前后嗜酸性粒细胞富集群中 TLR2、TLR4、TLR7 和 TLR9 的表达,SPSS软件分析TLR2+、TLR4+、TLR7+和TLR9+嗜酸性粒细胞的相关性.结果 与健康组比较,AR 患者未经过敏原刺激时嗜酸性粒细胞富集群中TLR2+细胞的比例降低4%,TLR4+细胞的平均荧光强度(mean fluorescence intensity,MFI)升高20%,TLR7+细胞的比例升高4.8 倍.蒿草花粉过敏原提取液诱导AR患者TLR2+嗜酸性粒细胞的比例升高7.8%.此外,AR患者血液 TLR2+和 TLR4+、TLR2+和 TLR7+、TLR7+和 TLR9+的嗜酸性粒细胞均呈中度相关(Pearson相关系数分别为r=-0.670,P＜0.01;r=-0.430,P＜0.05;r=0.446,P＜0.05).而健康人嗜酸性粒细胞富集群中TLR2、TLR4、TLR7 和TLR9 的变化在过敏原刺激前后差异均无统计学意义.结论 嗜酸性粒细胞源的 TLR2、TLR4 和TLR7 可能在 AR中起重要作用,TLR2 、TLR4 和TLR7 可能是治疗 AR的潜在靶点.%Objective To investigate expressions and correlations of TLR2,TLR4,TLR7 and TLR9 in eosinophil-enriched cell populations from patients with allergic rhinitis (AR),and elucidate their roles in AR. Methods Peripheral venous blood samples were collected from healthy controls (HCs)and AR patients,and then incubated with crude extracts of Artemisia pollen,dust mite,and Platanus pollen,respectively.Levels of TLR2 , TLR4,TLR7 and TLR9 in blood eosinophil-enriched cells were detected by flow cytometry.Correlations between TLR2+,TLR4+,TLR7+and TLR9+eosinophils were analyzed by SPSS.Results Levels of TLR2+eosinophils from patients with AR were reduced by 4%,mean fluorescence intensity (MFI)of TLR4+eosinophil was elevated by 20%,and TLR7+eosinophils increased up to 4.8 folds compared with HCs when cultured with medium only (P＜0.05).Artemisia pollen extracts induced approximately 7 .8 % of increase in TLR2+eosinophils from AR patients.In addition,correlations between TLR2+and TLR4+eosinophils,TLR2+and TLR7+eosinophils,and TLR7+and TLR9+eosinophils were -0.670 (P＜0.01),-0.430 (P＜0.05)and 0.446 (P＜0.05),respectively. However,allergens had few effects on TLR2,TLR4,TLR7 and TLR9 expressions in HCs.Conclusion Eosinophil-derived TLR2 ,TLR4 and TLR7 are likely to play a key role in AR.TLR2 ,TLR4 and TLR7 might become the potential targets for AR treatment.