竹节参药材化学成分指纹图谱研究

摘要

建立竹节参药材化学成分的指纹图谱，为竹节参药材的质量分析及其临床应用提供依据。采用HPLC法，C18色谱柱，以0．05％磷酸水溶液和乙腈为流动相，梯度洗脱，检测波长为203 nm，建立了竹节参化学成分指纹图谱。竹节参指纹图谱共形成19个共有峰，通过对照品比对和HPLC-MS／MS 分析，指认了10个共有峰，分别是人参皂苷Rg1、Re、Rb1、Rg2、Ro、Rg3、Rh2、竹节参皂苷Ⅳ、Ⅰb、Ⅳa。主成分分析和聚类分析将14批药材分为3类。主成分分析与聚类分析结果一致。所建立的竹节参化学成分指纹图谱准确、可靠、可行，对于竹节参药材的生产和质量控制提供依据。%To establish the chemical composition fingerprints for identification and evaluation of Rhizoma Panacis Ja-ponici.HPLC method was used to establish Rhizoma Panacis Japonici fingerprint to identify them with the mobile of acetonitrile -0.05%phosphoric acid water solution by gradient elution on an C 18 column at the detection wave-length of 203 nm.Twenty two common peaks were defined in Rhizoma Panacis Japonici by fingerprint analysis in all.Ten of them were identified as ginsenoside Rg 1、Re、Rb1、Rg2、Ro、Rg3、Rh2;Chikusetsu saponin Ⅳ,Ⅰb,Ⅳa by comparing with standard references or HPLC-MS/MS method .The samples of Rhizoma Panacis Japonici was classfied into 3 clusters by principal component analysis and cluster analysis .The HPLC fingerprint provides the ex-perimental basis for manufacture and quality control of Rhizoma Panacis Japonici .