以干燥菌剂中的活菌数为指标，通过单因素实验和正交实验对耐高温马克斯克鲁维酵母HY32-1的固体发酵工艺进行优化。结果表明，最佳培养基为：以麸皮97．3％、糖蜜1％、酵母粉0．5％、脱脂奶粉0．5％，尿素0．1％、KH2 PO40．5％、MgSO4·7H2 O 0．1％为发酵基质，并采用3倍浓度YPD加富培养液代替固体发酵培养基中20％水分。菌剂的最佳发酵和制备条件为：发酵时间30 h、发酵温度37℃、培养基含水率50％，发酵后干燥温度45℃。优化后所制备的HY32-1固体菌剂活菌数高达3．7×108 cfu／g，比优化前的活菌数提高了77．9％。%In order to improve the production of active cells by Kluyveromyces marxianus HY-32 , the proportion of medium component and the treatment conditions were optimized by single factor test and orthogonal test.The result showed that, a maximal yield of cell amount could be obtained in the enrichment medium, which contains 96.4%wheat bran, 1%molasses, 0.5%yeast extract, 0.1% urea, 0.5% KH2 PO4 , 0.1% MgSO4 · 7H2 O, and with 20%moisture replaced by three times YPD medium.The best treatment conditions were as follows: fermentation temperature 37 ℃, medium moisture 50%, culture time 30 h and drying at 45 ℃.Compared with the initial con-ditions, the optimized medium and treatment process resulted in a 77% increase in the active cells, which im-proved from 2.08 ×108 cfu /g to 3.7 ×108 cfu /g.
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