七彩红竹查尔酮异构酶基因 IhCHI1的克隆与表达分析

摘要

Chalcone isomerase ( CHI) is one of the important structure genes in Anthocyanins biosynthesis.IhCHI1 was successfully obtained from Indosasa hispida cv.‘Rainbow ’ by RT-PCR with special primers designed based on the transcriptome data , and this sequence accession number is KJ 477333 in GenBank.Sequence analysis showed that IhCHI1 had complete cDNA open reading frame ( ORF ) , which consisted of 690 bp encoding 229 amino acids.Blast analysis showed that IhCHI1 belonged to the CHI family.Phylogenetic analysis showed that Indosasa hispida cv.‘Rainbow’ had close relative rationship with Oryza sativa and Zea mays.IhCHI1 expression analysis in different organs of Indosasa hispida cv.‘Rainbow ’ by RT-PCR showed that IhCHI1 strongly expressed in young red culm.%查尔酮异构酶（ CHI）是花青素生物合成过程中重要的结构基因。本项实验根据转录组数据设计的特异引物，利用RT-PCR方法成功从七彩红竹红色幼茎中克隆到七彩红竹的查尔酮异构酶基因IhCHI1（ GenBank登录号为KJ477333）。序列分析结果表明， IhCHI1包含完整的cDNA开放阅读框（ORF），由690 bp组成，编码229个氨基酸。 Blast比对结果显示，该蛋白属于CHI家族蛋白；系统进化分析结果显示，七彩红竹与禾本科植物水稻、玉米等亲缘关系较近；利用RT-PCR方法对IhCHI1基因在不同组织中表达情况进行分析，结果表明， IhCHI1在七彩红竹的红色幼秆中表达量最高。