澳洲茶树组培继代芽瓶外生根技术研究

摘要

Based on the Melaleuca alternifolia subculture plantlet buds cultured for 40 days, the effect of different nursery substrate, growth regulators and transplanting month on ex vitro rooting of M.alternifolia were studied.The results showed that the best solution of ex vitro rooting of subculture plantlet buds of M.alternifolia was soaking buds (≥3cm)into 0.4mg/mL ABT solution for 30-50min, and dipping in homogenate which consist of corresponding hormone concentration solution mixed evenly with talcum powder, then transplant them in red subsoil in the win-ter.With this operation, the rooting rate was 92%, root numbers was 8.96, root length was 2.4cm on average. It will improve propagation efficient and reduce the cost of large scale production of M.alternifolia.%以培养40d的澳洲茶树组培继代芽为试验材料,研究不同基质、生长调节剂及处理方式和扦插时间对其瓶外生根效果的影响.结果表明,澳洲茶树瓶外生根的最佳方案是,在冬季,以红心土作为基质,将≥3cm的继代芽浸泡于0.4mg/mL的ABT溶液中30-50min,并蘸取相应浓度激素溶液与滑石粉的混合匀浆后扦插.此方案的生根率为92%,生根8.96条,平均根系长2.4cm,对促进澳洲茶树繁殖效率,降低生产成本具有重要意义.