Normal Human Astrocyte Instructions for Initiation of Cultures from Cryopreserved Cells and Subculture

摘要

This technical report outlines procedures that have been developed to provide a cost-effective way to produce large quantities of Normal Human Astrocyte (NHA) cells for studying the mechanism(s) of action of chemical warfare agents (CWAs) and medical countermeasures against CWAs. Five milliliters of a supplement provided by Clone Express was added to 467 mL of DMEM F12 media as well as 25 mL of fetal bovine serum plus 0.3 mL gentamicin and 2.5 mL of fungizone. Cell viability and cell proliferation were evaluated using the Coulter Z1 Particle Counter.