外源激素对桑树组培无菌苗继代增殖及生根的影响

摘要

Objective The purpose of this experiment was to investigate the effects of exogenous hormone factors on subculture multiplication and root induction of tis-sue-cultured seedlings in mulberry. [Method] The aseptic seedlings of Guisangyou 12 were used as the materials and the comparative experiment on subculture multi-plication and root induction used different kinds,concentrations and combinations of exogenous hormones. [Result] The hormone combination of 0.1 mg/L IBA+1.5 mg/L 6-BA+0.03 mg/L TDZ showed the best effects to bud multiplication, and the multiple of bud multiplication was 5.28. The effects of NAA was better than IBA and IAA on root induction. The hormone combination of 2.0 mg/L NAA+1.0 mg/L PP333 was most suitable to the root induction, in which the rooting rate arrived to 100%, the root number was 7.01 and the root length was 1.38 cm on average. [Conclusion] The results wil provide some technical reference for large-scale propagation of mul-berry seedlings in vitro.%[目的]探讨外源激素因子对桑树组培无菌苗继代增殖及生根的影响。[方法]以桂桑优12号为材料，从外源激素的不同种类、浓度及组合对桑树组培无菌苗继代增殖和生根诱导进行比较试验。[结果]以IBA 0.1 mg/L+6-BA 1.5 mg/L+TDZ 0.03 mg/L激素组合对芽增殖效果最好，芽增殖倍数达到5.28。 NAA对生根诱导的效果优于 IBA和 IAA；以 NAA 2.0 mg/L+PP331.0 mg/L的激素组合对生根诱导效果最佳，生根率达100%，根数为7.01条，根长为1.38 cm。[结论]该研究为桑树种苗的离体培养工厂化生产提供技术参考。