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EXO-BIOPOLYMER HAVING IMMUNO-MODULATING ACTIVITY FROM PHELLINUS PINI
EXO-BIOPOLYMER HAVING IMMUNO-MODULATING ACTIVITY FROM PHELLINUS PINI
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机译:具有桑树免疫调节功能的外源生物聚合物
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摘要
PURPOSE: Exo-biopolymer having immuno-modulating activity from Phellinus pini is provided, which exo-biopolymer has improved immuno-modulating activity such as antimutagenicity, nitric oxide formation, activation of lysosome in macrophage cells, and division of spleen cell, so that it can be useful for improving immunity of human. CONSTITUTION: The exo-biopolymer having immuno-modulating activity from Phellinus pini has molecular weight of 5 kDa, contains 89.74% of total saccharides and 10.26% of total protein, and has glucose as a main neutral saccharide. The method for isolating the exo-biopolymer having immuno-modulating activity comprises the steps of: centrifuging the Phellinus pini cultured medium at 10,447xg for 20 minutes to obtain the supernatant; adding ethanol into the supernatant in a ratio of 4:1 and reacting them for 24 hours to obtain the precipitate; dissolving the precipitate in distilled water, dialysis of the precipitate dissolved solution and freeze-drying the dialyzed solution.
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