4-松油醇型澳洲茶树组培生根技术研究

摘要

Melaleuca alternifolia is a kind of short cycle tree species for industrial material.The 4-Terpinol type M.ahemifolia which was selected according to the chemical constituents has great application prospect.Based on the results of mono-factor experiment that NAA and IBA were the beneficial elements during rooting,an orthogonal experiment was performed with WPM,B5,and MS as media.The rooting rate was 91.67％±2.72％ with in the medium of 1/2 B5 + NAA0.2 mg · L-1 +IBA0.5 mg· L-1,which was the best for rooting.The culture medium was proposed the main factor influencing the rooting rate through the direct analysis of the orthogonal experiment.The order of factors affecting on the rooting rate was culture medium ＞NAA concentration ＞IBA concentration according to the range analysis,and the optimal scheme was 1/2 B5+ NAA0.2 mg · L-1 +IBA0.2 mg · L-1,which was expected to provide better rooting performance.%澳洲茶树(Melaleuca alterni olia)是一种新兴的短周期工业原料林树种,根据枝叶成分定向选育出的4-松油醇型澳洲茶树更是具有极佳的生产应用前景.本研究首先通过单因素试验,确定了NAA和IBA 2种生长调节剂对4-松油醇型澳洲茶树单芽生根具有促进作用;随后选择WPM、B5、MS作为基本培养进行培养基和生长调节剂的正交试验.结果表明,1/2 B5+NAA0.2mg·L-1+IBA0.5 mg·L-1中生根率为91.67％±2.72％,根系发育状况较好,整体生根效果最好.对正交试验结果直观分析发现,培养基因素是影响生根率的主要因素.按照极差推测的影响因素排序为培养基＞ NAA浓度＞IBA浓度,其中最优方案为1/2 B5 +NAA0.2 mg·L-1+IBA0.2 mg·L-1,推测可以获得更好的生根效果.