We identified a NLR-C gene from carp (Cyprinus Carpio L. ) with RT-PCR and RACE, and analyzed the expression of this gene by qRT-PCR. In healthy carp,the transcript was detected with highest expression in brain and PBL,and higher in head kidney , gill, hindgut and oral epithelial. Analysis of expression of the NLR-C gene in carps after infection with Vibrio anguillarum reveales a significant up-regulation in liver,spleen,head kidney and hindgut tissues, suggesting that it is involved in the immune response of carp against the bacterial pathogen.%利用RT-PCR和RACE技术克隆得到鲤鱼(Cyprinus carpioL.)NLR-C基因cDNA全长.经qRT-PCR检测发现,NLR-C基因在鲤鱼血液和脑中表达最高,在头肾、鳃、后肠和口腔上皮中有较高表达.采用鳗弧菌(V.anguillarum)刺激后,NLR-C mRNA在肝、脾、头肾和后肠中的表达有不同程度升高.结果显示,NLR-C基因参与鲤鱼天然免疫应答过程,具有抵御病原微生物侵袭的作用.
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