GEN glycoprotein (G-GEN) RUSSIAN REFERENCE STRAIN ZL-4 VIRUS spring viraemia of carp and recombinant plasmid DNA PCDNA-G and PBACARPVAX-G, gene expression of glycoproteins and ensures the development of protective immunity in fish AGAINST virus infection spring viraemia of carps

摘要

1. on the surface u0442u0440u0430u043du0441u043cu0435u043cu0431u0440u0430u043du043du043eu0433u043e u0433u043bu0438u043au043eu043fu0440u043eu0442u0435u0438u043du0430 (g gene) of the spring u0432u0438u0440u0435u043cu0438u0438 carp u0440u0435u0444u0435u0440u0435u043du0441u043du043eu0433u043e u0434u043bu00a0 russia u0438u0437u043eu043bu00a0u0442u0430 zlotys - 4 with u043du0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043d spine, as set out in the u0444u0438u0433.2.;2. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna pcDNA g size 6961 sukhoy.and u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du043eu0439 mass 461106 dalton, u043eu0431u0435u0441u043fu0435u0447u0438u0432u0430u044eu0449u0430u00a0 expression of surface u0442u0440u0430u043du0441u043cu0435u043cu0431u0440u0430u043du043du043eu0433u043e u0433u043bu0438u043au043eu043fu0440u043eu0442u0435u0438u043du0430 virus spring u0432u0438u0440u0435u043cu0438u0438 carp u0440u0435u0444u0435u0440u0435u043du0441u043du043eu0433u043e u0434u043bu00a0 grew up the u0438u0437u043eu043bu00a0u0442u0430 zlotys - 4 under the control of cmv promoter or fish in cages in the animal u0432u043du0443u0442u0440u0438u043cu044bu0448u0435u0447u043du043eu043c introduction of dna vaccine, u0432u043au043bu044eu0447u0430u044eu0449u0430u00a0 EcoR i Xho i vector fragment dna u043fu043bu0430u0437u043cu0438 you pcDNA 3.1. (+) of 5405 sukhoy.[Invitrogen, usa], containing the cmv promoter and the sequence of BGH poly and that the expression of g gene in the cells u044du0443u043au0430u0440u0438u043eu0442; t7 promoter and the gene of resistance to the u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443, oh u0431u0435u0441u043fu0435u0447u0438u0432u0430u044eu0449u0438u0435 selecting and breeding target plasmid in the cells of the bacteria e. coli; EcoR i Xho i fragment size 1556 sukhoy.containing a full-size g gene of surface u0442u0440u0430u043du0441u043cu0435u043cu0431u0440u0430u043du043du043eu0433u043e u0433u043bu0438u043au043eu043fu0440u043eu0442u0435u0438u043du0430 virus spring u0432u0438u0440u0435u043cu0438u0438 carp u0440u0435u0444u0435u0440u0435u043du0441u043du043eu0433u043e u0434u043bu00a0 russia u0438u0437u043eu043bu00a0u0442u0430 zlotys - 4; genetic the markers, gene of resistance to the u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443; unique sites u0443u0437u043du0430u0432u0430u043du0438u00a0 u0440u0435u0441u0442u0440u0438u043au0446u0438u043eu043du043du044bu043cu0438 u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0430u043cu0438 with the following coordinates: EcoR i (722), Kpn i (691), Xho i (2278), Xba i (442).;3. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna pBACarpVax - g the size of 5437 sukhoy.and u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du043eu0439 mass 361106 dalton, u043eu0431u0435u0441u043fu0435u0447u0438u0432u0430u044eu0449u0430u00a0 expression of surface u0442u0440u0430u043du0441u043cu0435u043cu0431u0440u0430u043du043du043eu0433u043e u0433u043bu0438u043au043eu043fu0440u043eu0442u0435u0438u043du0430 virus spring u0432u0438u0440u0435u043cu0438u0438 carp u0440u0435u0444u0435u0440u0435u043du0441u043du043eu0433u043e u0434u043bu00a0 grew up the u0438u0437u043eu043bu00a0u0442u0430 zlotys - 4 under the control of promoter or - u0430u043au0442u0438u043du0430 carp fish in cages in the animal u0432u043du0443u0442u0440u0438u043cu044bu0448u0435u0447u043du043eu043c dna vaccine, u0432u043au043bu044eu0447u0430u044eu0449u0430u00a0 EcoR i Xho i vector u0444u0440u0430u0433u043cu0435u043d t dna plasmid pBACarpVax size 3881 sukhoy.[u043fu0440u043eu0438u0437u0432u043eu0434u043du0430u00a0 from pVax1 Invitrogen, usa), which is u0430u043au0442u0438u043du043eu0432u044bu0439 promoter carp Nru i restriction sites EcoR i and sequence BGH poly and that the expression of g gene in u043bu0435u0442u043au0430u0445 u044du0443u043au0430u0440u0438u043eu0442; t7 promoter, and the gene of resistance to the forces pMB1 u043au0430u043du0430u043cu0438u0446u0438u043du0443 for selecting and breeding target plasmid in the cells of the bacteria e. coli; EcoRI - XhoI fragment r u0430u0437u043cu0435u0440u043eu043c 1556 sukhoy.containing a full-size g gene of surface u0442u0440u0430u043du0441u043cu0435u043cu0431u0440u0430u043du043du043eu0433u043e u0433u043bu0438u043au043eu043fu0440u043eu0442u0435u0438u043du0430 virus spring u0432u0438u0440u0435u043cu0438u0438 carp u0440u0435u0444u0435u0440u0435u043du0441u043du043eu0433u043e u0434u043bu00a0 russia u0438u0437u043eu043bu00a0u0442u0430 zlotys - 4; genetic the markers, gene of resistance to the u043au0430u043du0430u043cu0438u0446u0438u043du0443; unique sites u0443u0437u043du0430u0432u0430u043du0438u00a0 u0440u0435u0441u0442u0440u0438u043au0446u0438u043eu043du043du044bu043cu0438 u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0430u043cu0438: EcoR i (1615), Xho i (3257).