Objective To monitor plasma rifampicin concentrations in patients with pulmonary tuberculosis by HPLC. Methods The assay was conducted on C18 GL sciences column (5 μm, 4.6 × 250 mm)with the mobile phase of the mixture of CH3OH-CH3CN-0.04mol/L KH2PO3(30:30:40)at a rate of 1.2 ml/min,the UV detection wavelength was 340 nm. The column temperature was 20 ℃. Results A good linearity was obtained from 0. 30 - 24 μg/ml with a correlation coefficient of 0. 999 5. The average recovery of the assay was 98. 87%. Among 140 patients administrated with 450 mg rifampicin orally or intravenously, the plasma drug concentration ranged 0. 50 - 12. 75 μg/ml in 126 cases, ranged 0. 30 - 0.5 μg/ml in 12 cases and was less than 0.3 μg/ml in 2 cases. Conclusion The method applied in this study is accurate and sensitive, which can be used to monitor plasma rifampicin concentration for rational drug administration in clinical practice.%目的 建立肺结核患者给予利福平后血药浓度的监测方法,为临床合理用药提供参考.方法 高效液相法,色谱柱为安捷伦C18柱,(5μm,4.6×250 mm);流动相为甲醇:乙腈:0.04 mol×L-1 KH2PO3( 30:30:40);流速1.2 ml/min,检测波长为340 nm;柱温20℃.结果 血浆中利福平在0.30 ～24 μg/ml范围内浓度与峰面积线性关系良好,r=0.9995,平均回收率为98.87％.140例肺结核患者静滴或口服利福平450 mg后,其中126例血药浓度检测结果在0.50～12.75 μg/ml之间,12例血药浓度检测结果0.30～0.5 μg/ml之间,2例血药浓度小于0.3μg/ml.结论 该方法准确、灵敏,专属性强,重现性好,适用于治疗药物监测,可为临床合理用药提供数据参考.
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