rhEPO对大鼠肠道缺血再灌注损伤的保护作用

摘要

Objective: To explore the protective effect of rhEPO on intestinal ischemia reperfusion in rats and observe its effects on the expression of tight junction protein (ZO-1). Methods: Thirty-six male Wistar rats were randomly divided into three groups, the sham operation group A, the ischemic/reperfusion group B, the rhEPO pretreatment group C, n=12 respectively. Laparotomy was operated in group A,rhEPO 1000 U/kg were injected subcutaneously 10 minutes before ischemia in group C, superior mesentery artery (SAM) were occlused for 45 minutes ischemia in group B and C, then followed reperfusion 2 hours. Serum diamine oxidase(DAO), D-lactate and TNF-α were measured and intestine histopathology was observed by HE staining and the expression of ZO-1 protein were detected by im-munohistochemistry. Results: Compared to group B, the arrangement of intestinal villus was better, the damage degree was insignificantly and the expression of ZO-1 protein was higher in group A and C. The serum concentration of DAO,D-lactate and TNF-α in three groups had difference significantly (P<0.05),and there was statistical significance (P<0.05) in group A and C. Conclusion: RhEPO protects the intestine from ischemia reperfusion injury in rats and have effects on the expression of ZO-1 protein.%目的:探讨人类重组促红细胞生成素(rhEPO)对大鼠肠道缺血再灌注损伤的保护作用,观察其对肠道紧密连接蛋白(ZO-1)表达的影响.方法:健康雄性Wistar大鼠36只,随机分为假手术组(A组)、缺血再灌注组(B组)和rhEPO预处理组(C组)3组,每组12只.A组只给予剖腹手术,C组缺血前10min预先皮下注射rhEP0 1 000 U/kg,B、C组均夹闭肠系膜前动脉45 min后恢复再灌注2h,取血清及小肠组织行二胺氧化酶(DAO)、D-乳酸、TNF-α检测及HE染色和ZO-1蛋白免疫组织化学检测.结果:与B组比较,A、C组小肠绒毛排列整齐,破坏程度明显减轻,ZO-1蛋白表达水平增高.ELISA结果显示:血浆DAO、D-乳酸和TNF-α水平在B组和A、C组之间均有显著性差异(P＜0.05),A和C组之间差异也有统计学意义(P＜0.05).结论:预先注射rhEPO对缺血再灌注损伤的肠道有保护作用,并且其对ZO-1蛋白的表达有一定影响.