Cloning and Functional Analysis of A NAC-Like Gene Csnac in Tea Plant (Camellia sinensis)

摘要

The plant-specific NAC(NAM,ATAF1 /2,CUC2) transcription factors play important roles in stess responses and devlopment in plants,but little was known about NACs in tea plants(Camellia sinensis).In this study,a fulllength cDNA sequence(CsNAC) was isolated by suppressive subtractive hybridization(SSH) in Ectropic oblique feeding-induced tea plants.Gene sequence analysis showed that the putative protein of CsNAC contained a conserved NAC domain,which is the typical characteristic of NAC transcription factors.qRT-PCR analysis revealed that CsNAC was suppressed by Ectropis oblique feeding and mechanical damage.Treatment of defence-related homones methyl jasmonate(MeJA) decreased the transcript abudance of CsNAC and salicylic acid(SA) could induce the expression of CsNAC.The transcript levels of CsNAC was down-regulated after applying abscisic acid(ABA) and the expression patterns of CsNAC were different by drought or cold stress.Subcellular localization analysis revealed that CsNAC protein was localized in the nucleus.These results suggested that CsNAC may play a role in defense against insects and adaptation to abiotic stresses.