[Objective] This study aimed to verify the feasibility of in silico cloning of functional candidate genes in tea. [Method]Theobroma cacao caffeine synthase gene BCS1 was used as a probe to search the established tea EST database using BLAST; 26 tea ESTs highly homologous to BCS1 were obtained,which were assembled using CAP( contig assembly program) of Bio Edit software; subsequently,two EST contigs harboring ORF were obtained,which were named TCSnew1 and TCSnew2,respectively. Nucleotide sequences and deduced amino acid sequences of theses two genes were compared with those of c DNA of tea caffeine synthase gene TCS in the Gen Bank database that was cloned with experimental biological method. A phylogenetic tree was constructed for homologous analysis of the deduced amino acid sequences of theses three genes. [Result] in silico cloning of functional candidate genes in tea using a homologous gene of distantly related species as a probe is a feasible technical means. [Conclusion] This study provided the basis for in silico cloning of other functional genes in tea.
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