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农业生物技术：英文版

农业生物技术：英文版

该刊主要报道来自于与农业相关的植物学、动物学、微生物学、土壤肥料、食品科学及林业、海洋学等学科的农业生物技术研究成果。
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农业生物技术：英文版 >2023年第5期

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  • 摘要：Red sandalwood( Pterocarpus santalinus) is the most precious species of Pterocarpus,and the long maturity period of its heartwood is an important factor limiting its development. The 1-year-old plants of P. macarocarpus were chosen as the rootstocks to graft red sandalwood with single-bud scions of 1-year-old shoots in spring. The 5-month survival rate reached over 80%,and the grafted plants grew well. Moreover,the grafting significantly promoted the formation and development of the heartwood of red sandlawood. At 18 ^(th) month after grafting,compared with the seedling plants,the diameter of heartwood was increased by 82. 8% and 29. 6%,respectively,and the ratio of heartwood increased by 64. 8% and 29. 6%,respectively. The propagation technology had outstanding practical value and scientific significance,which has not been reported at home and abroad.
  • 摘要：This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan( T. distichum × T. mucronatum).Using the tender stems of Zhongshanshan 301 as the explants,the effects of NAA,6-BA,IBA and KT on the induction of differentiation,proliferation and rooting were evaluated on MS or 1/2 MS medium. The results showed that Zhongshanshan can be proliferated via tissue culture. The combined use of NAA and 6-BA in MS medium greatly promoted the differentiation of Zhongshanshan explants,and the optimal medium was MS + 0. 2 mg/L NAA + 0. 4 mg/L6-BA. The optimal medium for the proliferation of differentiated buds was MS + 0. 3 mg/L NAA + 0. 4 mg/L 6-BA,on which the proliferation rate was up to 3. 8. 1/2 MS medium was more conducive than MS medium to the induction of rooting. The optimal medium for the rooting of tissue-cultured Zhongshanshan shoots was 1/2 MS + 0. 3 mg/L IBA + 0. 2 mg/L NAA.
  • 摘要：In vitro maturation of mammalian oocytes is a key step during in vitro production of mammalian embryos. Studies have shown that the level of oocyte maturation is positively correlated with embryo cleavage rate and blastocyst rate. However,reactive oxygen species( ROS) in vitro is one of the main influencing factors of oocyte maturation. The results indicate that the addition of antioxidants can effectively improve in vitro oocyte maturation level and reduce the adverse effects of reactive oxygen species,which contribute to oocyte development. This paper summarizes research progresses on the effects of different antioxidants on in vitro development of mammalian oocytes,which is conducive to further analysis of the mechanism of action.
  • 摘要：As the main environmental factor of ginseng growth,illumination plays an important role in ginseng( Panax ginseng) growth and development. Although ginseng is a shade-demanding plant,we need to get higher yield and quality under ideal light conditions. Three different illumination conditions for growth were created through different shading treatments. It was concluded that the adjustment of illumination according to monthly irradiance is beneficial to ginseng growth; appropriate illumination significantly affected root diameter; and SPAD value could also be used in the production of ginseng,but different light environments have influences on its accuracy. Reasonable light adjustment could improve the accuracy of the prediction value of SPAD.
  • 摘要：The 21^(st) century is the century of Biology. With the rapid develop of modern biotechnology,GM Food will play a great role in people's life. This article shows the prospective vision of the world 100 years later.
  • 摘要：In this study,chalcone synthase and chalcone isomerase encoding genes( GhCHS and GhCHI) were cloned from upland cotton( Gossypium hirsutum)cultivar HM-40,and analyzed bioinformatically. Their functions were analyzed through virus induced gene silencing( VIGS). The results showed that GhCHS gene has an open reading frame( ORF) of 1 170 bp and encodes a protein of 389 amino acids. Many phosphorylation sites were detected in GhCHS protein,suggesting that it may be involved in kinase phosphorylation. The deduced GhCHS protein was most closely related to Theobroma cacao CHS protein according to phylogenetic analysis. The GhCHI ORF was 630 bp and encoded a protein of 209 amino acids. Many phosphorylation sites were found in GhCHI protein,indicating that it may be related to kinase phosphorylation. The GhCHI protein was most closely related to Hibiscus cannabinus CHI protein according to phylogenetic analysis. Quantitative PCR( q PCR) showed that GhCHS and GhCHI were rapidly activated after inoculation with Verticillium dahliae VD07,and then their expression levels kept increasing over time,indicating that the two genes might play an important role in the defense response against Verticillium wilt. Virus induced gene silencing( VIGS) was used to silence endogenous GhCHS and GhCHI genes in upland cotton plants before VD07 was inoculated to identify disease resistance. The results showed that the disease index of plants untreated with Agrobacterium tumefaciens was 31. 2,and that of the plants inoculated with empty vector was 30. 0. The disease index of GhCHS-silenced plants was 72. 5,and that in GhCHI-silenced plants was 67. 5. These results confirmed that GhCHS and GhCHI may play an important role in defense response of upland cotton to Verticillium wilt.
  • 摘要：In this paper,by analyzing the genetic diversity of cultivated soybean germplasm resources in China,the environmental and genotypic factors that affect the genetic diversity of cultivated soybean germplasm resources were explored to further expand the genetic basis of the existing germplasm resources of cultivated soybean in China. Moreover,research progress on genetic diversity of cultivated soybean in China was summarized,which not only revealed the geographical characteristics of genetic diversity of cultivated soybean in China,but also proposed direction for research of genetic diversity of soybean.
  • 摘要：Guitang 46 is an excellent sugarcane variety bred by the Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences under the " FiveNursery" breeding program through regional trials and production experiments,which exhibits high yield,stable yield,and various excellent characters such as medium maturing,high sucrose content,good ratoon ability,more effective stems,uniform growth and strong resistance to smut. Moreover,Guitang 46 has strong lodging resistance and good defoliation ability,which is suitable for both mechanical management and harvesting,and manual operations. Therefore,Guitang 46 is a new variety with high promotion potential. In June 2015,Guitang 46 was approved by Guangxi Crop Variety Approval Committee[GSZ2015001]. At present,Guitang 46 has been cultivated within a certain area.
  • 摘要：This study was conducted to investigate the effects of long-term located fertilization on soil phosphorus,the changes of soil available phosphorus( OlsenP),the evolution of soil total phosphorus( TP) and the ratio change of Olsen-P to TP( PAC) by 33-year fertilization experiments in winter wheat-summer maize rotation system in Shandong fluvo-aquic soil. Eight treatments were designed as no fertilization( CK),nitrogen fertilizer( N),nitrogen and phosphate fertilizer( NP),nitrogen and potassium fertilizer( NK),phosphate and potassium fertilizer( PK),nitrogen-phosphate-potassium fertilizer( NPK),reduced NPK fertilizer( N_(15) PK),and increased NPK fertilizer( N_(25) PK). Meanwhile,eight organic fertilizer-added treatments were designed based on the application of inorganic fertilizer the same as the above ones. The results showed that TP,Olsen-P and PAC of treatments added with organic fertilizer were higher than those without organic fertilizer,and those of the treatments applied with phosphate fertilizer were higher than those of no phosphate fertilizer. With the increase of years,soil P pool decreased due to crop absorption,nutrient loss and morphological transformation and other causes under the treatments of without and only phosphate fertilizer,while remained stable under the treatments added with organic fertilizer. The PAC values were generally lower in fluvo-aquic soil,and it could be improved by the application of organic fertilizer. On the whole,the application of chemical phosphate fertilizer combined with organic fertilizer could improve the phosphorus content in soil and ensure the supply of phosphorus nutrition. This study would provide scientific basis for fertilization management and soil fertility in fluvo-aquic soil.
  • 摘要：According to the data of analytic trees,an empirical equation of tree growth was constructed,with annual growth as variable and time and annual precipitation as independent variables. Through arithmetical operation including derivation of function and so on,the effect of precipitation on tree growth was studied through the rejection of effect of time factor.
  • 摘要：In order to obtain cytogenetic data of Odontobutis potamophila,head-kidney cells were collected as experimental materials to prepare chromosome specimen. The karyotypes of O. potamophila and the distribution and amount of transcriptionally active nucleolar organizer regions on chromosomes were analyzed by Giemsa staining and Ag-NORs. The results showed that the number of diploid chromosomes of O. potamophila was 44; the genome of O. potamophila was composed of 44 telocentric chromosomes; the karyotype formula was 2 n = 44 t,NF = 44; Ag-NORs were found in the paracentromeric region of chromosome. The results may lay the foundation for revealing the genetic pattern and chromosomal evolution of O. potamophila and contribute to further genetic breeding of O. potamophila.
  • 摘要：Tianfu No. 1 is bred from a plant in the population of Sichuan through system selection,which is characterized with early and uniform germination,hypertrophic shoots,full cover with pekoes and long picking period of single shoots. Compared with those of Fuding white tea( CK),the fresh leaf yield,tea polyphenols content,free amino acids content,caffeine content and soluble sugar content of Tianfu No. 1 were increased by 8. 89%,6. 98%,10. 00%,2. 96% and31. 58%,respectively. Tianfu No. 1 is a new rare breakthrough tea variety with high development potential,good intrinsic biochemical quality,and strong adaptability for processing multi-typed tea products such as green tea,white tea,black tea and dark tea,etc. It has strong stress resistance and can still grow better in low-humidity areas. In addition,it is not easy to flower and fruit.
  • 摘要：To evaluate the extraction process of Huidouba polysaccharides( HDBP) and its anti-diabetic activity,this study focused on the two contents as follows:extraction process of HDBP,which was optimized by orthogonal experiments using the extraction ratio of HDBP for the index; and bioactivity evaluation of HDBP on streptozotocin-induced diabetic rat model based on the histological,biochemical and enzyme linked immunosorbent assay( ELISA) method for histological observation,physiological and biochemical index such as fasting blood glucose( FBG),nitric oxide( NO),malondialdehyde( MDA),super oxide dismutase( SOD) and insulin level detection in pancreas tissue respectively. The results showed that HDBP extraction ratio was the highest under following conditions: the extraction temperature at 90 ℃,the extraction time of 1. 5 h,and the solid-liquid ratio at 1: 10. Compared with the normal control group and the model group,histological observation,physiological and biochemical index and insulin level in pancreas tissue significantly increased and FBG significantly decreased according to the dosage administrated. HDBP affected Streptozotocin-induced diabetic rats.
  • 摘要：Yuanliangyou 1000 is new combination of semilate rice bred by Yuan S and R900 by Institute of Grain and Oil Crops of Nanchang Academy of Agricultural Science,Yuanshi Seed Industry High Tech Co.,Ltd.and Jiangxi Pusheng Agricultural Development Co.,Ltd.It passed through new variety certification of Jiangxi Province in 2017,and approval number was Ganshendao 20170020,which was suitable for plantation of semilate rice in Jiangxi.In this paper,breeding process,combination characteristic,main cultivation technique and seed production technology of Yuanliangyou 1000 were introduced.
  • 摘要：[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C18 MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromatographic column and acetonitrile-0.4% phosphoric acid solution( 9∶91) as mobile phase.The separation was performed at a flow rate of 1.0 ml/min and a column temperature of 30 ℃,and the detection wavelength was set at 327 nm.[Results]Chlorogenic acid had a good linear relation in the range of 0.254 7-2.547 5 μg( R2= 0.999 9).The recovery rate was 97.8%,RSD = 1.82%( n = 9).[Conclusions]The content of chlorogenic acid in M.pubescens was determined by ultrasonic extraction and HPLC.The method was simple,stable and reliable and could be used for the quality control of M.pubescens.
  • 摘要：Sweet potato virus disease is a serious biological threat to sweet potato,which seriously affects the development of sweet potato industry in China.This paper gives a brief introduction to the main defensive measures of sweet potato virus disease,such as cutting of virus infection source,killing of viral transmission media,cultivation and application of virus-free sweet potato,use of antiviral agents,breeding of varieties with virus resistance,and mild strain cross protection,so as to provide some reference for this field.
  • 摘要：Newcastle disease( ND) is one of the most serious infectious diseases that infect the poultry industry.There is only one serotype of Newcastle disease virus( NDV),but NDVs can be divided into two distinct classes( class Ⅰ,and class Ⅱ) according to their genetic relationship.To develop a method for rapid quantitative detection of class Ⅰ NDV,a pair of primers and a TaqM an probe were designed and synthesized according to the conservative sequence of NP gene of class Ⅰ NDV.The positive recombinant plasmid harboring NP gene of JS-18-05 isolate was used as a positive template to establish the standard curve.A real-time fluorescent quantitative RT-PCR method was established for rapid detection of class Ⅰ NDV with strong specificity,high sensitivity and good repeatability.The established method exhibited a good linear relationship within the concentration of 102 to 108 copies of NDV,by which 1 μl of 10 copy of NDV nucleic acid could be detected in the initial template.Compared with conventional virus isolation methods,the established method had similar sensitivity and led to the same results in detecting33 class Ⅰ,class Ⅱ NDV isolates.The study provided the basis for rapid quantitative detection of class Ⅰ NDVs and further clarification of their pathogenicity and pathogenic mechanism in poultry.
  • 摘要：In view of Melilotus officinalis having high protein content and significant effect on improving saline alkali soil,a comprehensive experiment about improving saline alkali soil by planting M.officinalis was carried out to study its effects of potential equivalent land and equivalent grain.The results showed that the forage yield was 80 000 kg/hm^2 in the degraded grassland field(average pH of 9.06)after planting M.officinalis.The forage had a crude protein content of 17.50%,so the total content of crude protein was 1 400.64 kg/hm^2,which was equal to 15.562 t of corns produced from 3.557 hm^2 of medium-quality arable lands.Similarly,it was equal to 3.573 t of soybeans produced in 1.786 hm^2 of medium-quality arable lands.Therefore,based on such principle,the comprehensive development of creatures-soil system can acquire the equivalent land and equivalent grain resources.
  • 摘要：In order to select the long-acting,low toxic,low-risk and multi-functional new pesticides for the control of sugarcane borders and woolly aphids and precise and efficient application technology,the control effect of 10%monosultap·thiamethoxam granular formulation and 1%Bt·clothianidin granular formulation on sugarcane borders and woolly aphids were studied.The results showed that 10%monosultap·thiamethoxam GR and 1%Bt·clothianidin GR had good control effects on sugarcane borders and woolly aphids.They were ideal slow-release,long-acting,low toxic and multi-functional new pesticides used to control sugarcane borders and woolly aphids.They could be used alternately with other pesticides to delay the emergence and development of pest resistance to pesticides.The best dosage of the two pesticides in the field was 45 kg/hm 2.They could be mixed with fertilizer(1 200-1 800 kg/hm 2),scattered in sugarcane ditches or at the base of sugarcane plants,and covered with soil or film from January to July.The control effect on dead heart seedlings damaged by borers could be up to above 79.2%,and the control effect on sugarcane woolly aphids could reach more than 98.8%.In comparison with the control group,the actual yield and sugar content of sugarcane increased by above 41 555 kg/hm 2 and 6.5%respectively.The application of slow-release,long-acting,strong systemic and multi-functional new agents with fertilizer around roots is convenient,precise and efficient,labor-saving,time-saving and environmentally friendly,and is worthy of being widely applied in sugarcane areas.
  • 摘要：Aflatoxins(AFs)are a major pollution source of grain pollution and are secondary metabolites produced by Aspergillus flavus and A.parasiticus,which are one of the most toxic and carcinogenic substances.Feeding animals with aflatoxin-contaminated food can cause toxicosis,acute liver injury or liver cancer in animals,and also can cause multiple organ damage,decline in production performance and disease resistance,even death,which brings great economic losses to animal husbandry.In addition,AFs can do great harm to the human body.When the body ingests large amounts of AFs,it may suffer from acute poisoning and acute liver damage;and AFs can cause chronic poisoning of the body with continuous intake of trace AFs.This paper briefly analyzed the effect of AFs on the structure and function of poultry immune organs,immune gene expression,immunosuppression and so on.
  • 摘要：The annual dynamic changes of nutrient content in soil and leaves of adult Areca catechu trees in the A.catechu producing area Tunchang were studied by fixed point observation method to provide the scientific basis for the rational fertilization of A.catechu.The results showed that the soil of the A.catechu plantation was weakly acidic on the whole,and soil pH was 5.53.Soil phosphorus content was extremely low,only 5.46 mg/kg.Nitrogen content in A.catechu leaves was the highest(up to 20.24 g/kg),while phosphorus content was the lowest(only 0.31 g/kg).The differences in nutrient content were related to the characteristics of nutrient demand.There was a difference in nutrient content of A.catechu leaves during the annual growth period.Because the maximum nutrient requirement period of A.catechu trees was from April to September,the important fertilization period of A.catechu trees was also from April to September.
  • 摘要：The cultivation of the economic crop flue-cured tobacco is greatly impacted by natural climates,making it a risk industry.In recent years,flue-cured tobacco production is influenced by extreme weather such as typhoon,low temperature,hail,frost,drought,plant diseases and insect pests.It is imperative to establish a sound insurance system for tobacco planting.In this paper,the practice and functions of tobacco planting insurance in Qianxinan Prefecture were summarized,the experience and existing problems in the practice process were analyzed,and finally the countermeasures and suggestions were proposed to further improve the tobacco planting insurance.
  • 摘要：[Objectives]This study was conducted to develop a type of hard biscuit using water chestnut flour and thus to expand the application of water chestnut.[Methods]Hard biscuits were made from water chestnut flour and wheat flour.Then,their sensory quality indices were evaluated,and the optimal formulation of the biscuits was determined through single factor test and orthogonal array testing.[Results]The proportion of water chestnut flour was the most important factor that influences the sensory quality of hard biscuits,followed by the proportions of sugar,milk powder and baking powder.The optimal formula of the hard biscuit was adding 9.0 g water chestnut powder,8.0 g sugar,0.5 g baking powder,and 3.6 g milk powder to every 100.0 g wheat flour.The sensory quality score of the water chestnut hard biscuits made according to this formula was 86.0.And these biscuits were complete in shape,uniform in color,crisp and rich in nutrients.[Conclusions]The study enriches the types and improves the nutritional value of hard biscuits,which provides a new idea for the application of water chestnut.
  • 摘要：[Objectives]An acute toxicity test of Miantan Fuzheng Powder was conducted on rabbits,which were given Miantan Fuzheng Powder at its maximum administration dosage and observed for 14 d,so as to provide reference for medicine development and clinical test.[Methods]New zealand rabbits were divided into four groups randomly,each of which included 4 rabbits,half male and half female.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups were applied with Miantan Fuzheng Powder once at a dose of 5 g/kg body weight,and the blank control group-intact skin and blank control-damaged skin groups were given the auxiliary material(vaseline)once.The rabbits were observed continuously for 14 d after the administration,and variation in body weight was recorded during the observation and then statistically analyzed with SPSS.After the 14 d of observation,the animals were killed through pentobarbital anesthesia and anatomized to observe main visceral organs including heart,liver,spleen lung and kidney.If toxic effect or organ abnormality exists,pathological sections would be prepared and observed.[Results]Rabbits in the blank control and Miantan Fuzheng Powder groups all showed normal appearance,behavior,breath state,position and posture,response to stimulation and growth of body weight,and no irritative erythema and edema on the skin,and the damaged skin recovered naturally within 14 d.No obvious abnormality was observed on visceral organs in autopsy.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups had no significant differences in body weight from the blank control group-intact skin and blank control-damaged skin groups before administration,and on the 7 th and 14 th day after administration.[Conclusions]In this acute toxicity test,the Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups were given the drug at the maximum administration dosage of 5 g/kg body weight,which was about 119 times of the clinical dose for human,and no obvious toxic effect was observed.
  • 摘要：Air pollution has become the most concerned regional environmental problem in China at present.According to the data of typical cities in Shandong Province from January,2014 to December,2015,the air quality status,pollution status and spatial variation characteristics of air pollutants were classified and analyzed,thereby providing a scientific basis for air pollution control.The conclusions of this study are as follows:(1)PM 2.5 was the most important atmospheric pollutant in Shandong Province;(2)Qingdao had the best air quality with a good air quality rate of 73.7%,while Jinan had the worst air quality with a good air quality rate only of 31.3%;and(3)the days with heavy pollution were mainly concentrated in winter,mainly due to coal-fired heating in winter.
  • 摘要：[Objectives]The ecological environment of orchard has a direct impact on fruit quality,and straw mulching can effectively improve the physicochemical indicators of orchard soil,so as to achieve environmental protection and yield increase.[Methods]Tests were carried out for 3 consecutive years of mulching straw in vineyard of southern Jiangsu Province.[Results]The results showed that mulching straw continuously could effectively improve soil physicochemical properties,weed controlling effect,and the photosynthetic rate of leaves,as well as the yield and quality of grapes.The contents of total nitrogen,available phosphorus,quick-acting potassium and organic matter increased up to 91.67%,259.76%,442.39%and 34.30%,respectively.Soil bulk density decreased by 7.15%,and the weed plant control effect reached 87.45%.The increased of fresh weight was up to 93.55%,and the increase of the leaf parenthetical rate was up to 18.24%.The increases of soluble solids content and single grain weight reached up to 8.82%and 12.75%.The rate of dehiscent fruit was decreased by 50.89%.The grape could pick for sale 3 days ahead,and the increase of unit area yield reached up to 13.16%.[Conclusions]Straw mulching cultivation in vineyard is a good model for efficient recycling of crop straw and safe production of quality grape,and it provides a new technical approach for straw utilization,promising with good economic and ecological benefits.
  • 摘要：The tests of raising seedlings of Moringa oleifera were carried out in autumn and spring in Chongqing.The results showed that in the case of container seedling raising,the seedling emergence rate in autumn was 90%.Due to the large humidity and extreme weather,and the limited management conditions such as ventilation,the seedlings suffered from serious diseases,and the preservation rate was only 30%.The emergence rate was 91%in the spring of 2017,and the seedlings had unanimous growth.However,the seedling preservation rate dropped to 75%due to hail.In the spring of 2018,the emergence rate was 93%,and the seedlings also had unanimous growth.By the end of May,the seedlings had grown to 15 cm high.In addition,in the field seedling raising in 2017 and 2018,the seedlings showed unanimous growth with the emergence rate of 88%and 90%,respectively,and the seedlings grew into 30 cm high with good growth conditions in May.
  • 摘要：In this study,Hy 322 gene was cloned from Vibrio alginolyticus.The total length of its gene was 969 bp,and it could encode 322 amino acids.The physicochemical properties,protein structure,genetic evolutionary relationship and antigenic characteristics of the effector protein Hy322 of V.alginolyticus HY9901 typeⅢsecretion system were studied and analyzed by bioinformatics methods and tools.The results showed that Hy322 is an unstable hydrophilic and acidic protein without a transmembrane region and a signal peptide,and secondary structure toα-helix.The evolutionary analysis showed that V.alginolyticus HY9901 and V.harveyi were clustered together,which indicated that the genetic relationship between the two species was closest.HY322 contains a FliN super family conserved domain associated with Flagellar motor switch.Bioinformatics analysis showed that the B-cell preponderant epitopes of Hy322 might be localized in the regions of 32-33,100-102,138-140,215-216,235-238 and 246-249.The 3D structure model of Hy322 subunit was simulated by SWISS-MODEL software and itwas found that the yscQ of Yersinia were similar and the similarity was 42.25%.In this study,the feasibility of Hy322 as a common antigen of Vibrio was verified from the perspective of bioinformatics,which laid the foundation for the next step in vaccine development.
  • 摘要：The composition of high molecular weight glutenin subunits(HMW-GS)was analyzed using SDS-PAGE with 70 Longdong dryland winter wheat germplasm resources as experimental materials.The results showed that Longdong dryland winter wheat germplasm resources had N,7+8,2+12 as the dominant HMW-GS composition(42.86%),while the dominant HMW-GS composition in the introduced germplasms was N,7+9,2+12(36.84%).On the Glu-B1 locus of Longdong dryland germplasms,subunit 7+8 had the highest frequency,accounting for 65.71%,and on the Glu-B1 locus of the introduced germplasms,subunit 7+9 had the highest frequency,accounting for 57.89%.Whether subunit 7+8 is related to drought resistance in varieties still needs further study.On Glu-D1 locus,high-quality subunit 5+10 appeared in the introduced resources for 5 times,accounting for 13.16%of all the introduced lines,and appeared in Longdong dryland winter wheat germplasm resources for 2 times,only accounting for 2.86%of all the resources,suggesting that Longdong dryland germplasms lack high-quality subunits and need further improvement.
  • 摘要：Digitaria sanguinalis is an annual worst weed in the grass family that occurs in all parts of China.It mainly does harm to dryland crops and direct-sowing rice fields.In agricultural production,chemical herbicides are mainly used to control them,but the problems of environmental pollution and drug resistance caused by chemical herbicides have become more and more severe,which is in urgent need of integrated management.In this paper,the biological characteristics,chemical control,microbial control and allelopathy of D.sanguinalis were reviewed.

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