茶假眼小绿叶蝉抗联苯菊酯品系和敏感品系解毒酶活性及增效作用研究

摘要

Tea green leafhopper, Empoasca vitis, is the most serious pest in tea gardens. The detoxifying enzyme activities and synergism in the bifenthrin-resistant and susceptible leafhopper were measured via biochemical methods and bioassays to clarify their potential relationship to the resistance of tea leafhopper to bifenthrin. The detoxifying enzyme activity tests showed that the activities of carboxylesterase (CarE), Cytochrome P450-mediated O-demethylation activity toward p-nitroanisole (PNOD) and glutathione-S-transferase (GST) in the resistant strain were all higher than those in susceptible strain. The activit ies of the three detoxifying enzymes in bifenthrin-resistant strain were 2.09, 4.34 and 1.59 folds those of the susceptible strain respectively. Synergism tests showed that the synergist ratios of triphenyl phosphate (TPP), piperonyl buoxide (PBO) and diethyl maleate (DEM) were 1.04, 1.09, and 1.00 folds respectively in the bifenthrin-susceptible strain and 1.80, 7.97 and 1.03 foldsrespectively in the bifenthrin-resistant strain. These results indicate that CarE and PNOD play important roles in bifenthrin resistance of tea leafhoppers.%为了明确茶假眼小绿叶蝉（Empoasca vitis）对联苯菊酯代谢抗性相关的解毒酶，本研究采用室内生物测定和生化分析方法，比较了假眼小绿叶蝉抗联苯菊酯品系和敏感品系的3种解毒酶活性差异，并分析了3种增效剂对联苯菊酯的增效作用。解毒酶活性测定表明，茶假眼小绿叶蝉抗联苯菊酯品系羧酸酯酶（CarE）、细胞色素 P450氧化酶 O-脱甲基（PNOD）、谷胱甘肽-S-转移酶（GST）比活力都高于敏感品系，3种解毒酶活性分别为敏感品系的2.09倍、4.34倍和1.59倍。增效剂磷酸三苯酯（TPP）、增效醚（PBO）和顺丁烯二酸二乙酯（DEM）在茶假眼小绿叶蝉敏感品系中的增效比分别为1.04、1.09、1.00，而在联苯菊酯抗性品系中的增效比分别为1.80、7.97、1.03。上述结果证明，羧酸酯酶和细胞色素 P450氧化酶活性的增强在茶假眼小绿叶蝉对联苯菊酯的抗性产生中起到了重要的作用。