茶尺蠖幼虫取食提高茶树儿茶素代谢响应强度

摘要

研究了茶尺蠖幼虫为害茶树叶片对儿茶素合成途径的影响.采用3龄茶尺蠖幼虫取食茶树新梢芽下第二叶,测定了儿茶素合成相关基因的表达水平和儿茶素含量.研究结果表明,与对照相比,茶尺蠖幼虫为害后3、6、12 h显著诱导了CsANR基因的相对表达水平,且在为害后3 h和12 h达到了极显著差异.CsLAR基因在茶尺蠖为害后6 h 和12 h,与对照具有显著差异.茶尺蠖幼虫为害后24 h 显著诱导了没食子酸、没食子儿茶素、表儿茶素、表没食子儿茶素没食子酸酯和表儿茶素没食子酸酯含量的升高,而茶尺蠖为害后48 h 仅没食子酸和没食子儿茶素的含量显著高于对照;儿茶素、表没食子儿茶素和没食子儿茶素没食子酸酯在茶尺蠖为害后24 h和48 h均没有被显著诱导.上述结果表明茶尺蠖幼虫为害提高了茶树儿茶素合成途径的代谢强度和儿茶素类化合物的积累.%In this study,the effect of feeding by Ectropis obliqua on the catechin pathway of tea plant was analyzed. The transcriptional levels of catechin-related genes and the contents of individual catechins in the infested or intact leaves were measured.The transcriptional level of CsANR in the infested leaves was significantly higher than that in the intact leaves 3,6 h and 12 h after infestation.Meanwhile,the infestation of E.obliqua also significantly induced the expression level of CsLAR after 6 h and 12 h. The contents of gallic acid, gallocatechin, epicatechin, epigallocatechin gallate and epicatechin gallate were significantly induced 24 h after infestation. Moreover, the contents of gallic acid and gallocatechin were also significantly induced 48 h after infestation. However, the infestation of E.obliqua didn't induce the increases of catechin,epigallocatechin and gallocatechin gallate.In a word, the infestation of E.obliqua affected catechin metabolism in tea plant.