星形胶质细胞上调基因-1 表达与食管鳞状细胞癌预后的相关性研究

摘要

目的:探讨食管鳞状细胞癌组织中星形胶质细胞上调基因-1 ( AEG-1 )的表达情况及其与临床分期、预后的相关性. 方法:应用半定量RT-PCR、Western blotting 及免疫组织化学技术检测AEG-1基因在食管鳞状细胞癌组织中的表达情况,并与肿瘤临床分期特征及预后进行相关性分析. 结果:食管鳞状细胞癌组织中AEG-1 mRNA及蛋白表达量分别为癌旁正常组织的8.2~18.5倍及4.6~13.2倍. 76例食管鳞状细胞癌石蜡包埋组织中AEG-1 蛋白表达阳性占70例. AEG-1在男性患者中的表达强度强于女性患者,在中晚期病变中的表达强度强于早期病变,另AEG-1表达强度与患者生存时间呈负相关. 结论:AEG-1不仅可以用于预测食管癌患者预后,也可能成为一个潜在的基因治疗靶点.%Objective: To investigate the expression of astrocyte elevated gene-1 ( AEG-1 ) in esophageal squamous cell carcinoma tissue and its correlation with the clinical staging and prognosis .Methods:We used semi quantitative RT-PCR, Western blotting and immunohistochemistry to detect AEG-1 gene in esophageal squamous cell carcinoma tissue and different levels of expression , and analyze relationship with clinical tumor stage characteristics and prognosis .Results: The esophageal squamous cell cancer of AEG-1 mRNA and protein expression were 8.2-18.5 times and 4.6-13.2 times respectively those of adjacent normal tissues .Among 76 cases of esophageal squamous cell carcinoma AEG-1 in paraffin embedded tissue protein expressed in 70 cases.The expression intensity of AEG-1 was higher in male patients than female patients , as well as in advanced lesions than in early lesions, whereas its expression was negatively correlated with the survival time of the patients . Conclusion:Not only can the expression of AEG-1 in esophageal cancer predict the prognosis of patients , but can also be used as a potential molecular target for gene therapy .