杜仲雄花氨基酸主成分分析与综合评价

摘要

【目的】研究不同种质杜仲Eucommia ulmoides雄花氨基酸含量差异,评价杜仲雄花基于氨基酸构成的综合品质。【方法】利用自动氨基酸分析仪对193份杜仲种质雄花氨基酸含量及组成进行测定,采用主成分分析法对杜仲雄花氨基酸进行客观评价,计算综合得分及排名。【结果】193份杜仲种质雄花中均含有17种氨基酸,总氨基酸和必需氨基酸质量分数分别为206．23和68．37 mg·g-1,天冬氨酸和谷氨酸是主要的2种氨基酸。经主成分分析提取了6个主成分,且第1和第2主成分(必需氨基酸因子)对杜仲雄花氨基酸影响较大。按5%入选率确定了氨基酸性状优良的6种雄花资源,综合评价得出9个种质(10419x、10519x、10345x、10444x、10370x、10552x、10589x、10339x和10515x)杜仲雄花氨基酸品质较好。【结论】主成分分析法可以有效比较不同种质杜仲雄花氨基酸综合质量。%Objective] To study the difference in amino acid content among male flowers of different Eucommia ulmoides germplasm, and evaluate the comprehensive quality of E. ulmoides male flowers based on amino acid composition.[Method]The contents and compositions of amino acids in male flowers of 193 E. ulmoides germplasm resources were determined by automatic amino acid analyzer, then the qualities based on amino acid contents were evaluated objectively by principal component analysis, and overall scores and rankings were calculated. [Result]There were 17 kinds of amino acids in male flowers of 193 E. ulmoides germplasm resources. The contents of total amino acids and essential amino acids were 206. 23 and 68. 37 mg·g-1 respectively, among which aspartic acid and glutamic acid were the two major amino acids. Six principal components were extracted, and the first and second principal components ( factors of essential amino acids ) had relatively high influence on amino acid contents in E. ulmoides male flowers. Six types of E. ulmoides germplasm with superior amino acid traits of male flowers were chosen by selecting the top 5%, and the male flowers of nine E. ulmoides germplasm resources including 10419x, 10519x, 10345x, 10444x, 10370x, 10552x, 10589x, 10339x and 10515x were evaluated as having the best quality based on their amino acid compositions. [Conclusion]The principal component analysis could be used to analyze the comprehensive quality of male flowers among diverse E. ulmoides germplasm based on amino acid compositions.