【目的】采用ISSR分子标记鉴别楠属3个树种。【方法】以紫楠、浙江楠和桢楠木材为对象，比对改良CTAB-SDS法、CTAB法和试剂盒法3种方法提取的木材基因组DNA质量，采用ISSR-PCR技术对3个树种24个样本基因组DNA进行扩增。【结果】改良CTAB-SDS法和CTAB法较适合这3种木材的DNA提取。序列扩增筛选出7条稳定、条带清晰且多态性好的引物，确定其最佳退火温度。7条引物共扩增出67条大小为100~2000 bp的条带，其中多态性条带43条，多态率64.2豫。【结论】这7条引物扩增的多态性条带都可用于鉴别与区分这3种楠木。%Objective]The aim of the study was to identifying 3 p hoe be species by inter simple se-quence repeat(ISSR)technique.[Method]The optimized CTAB-SDS,CTAB and kit for DNA extrac-tion methods were used to extract wood DNA from P. sheareri,P. chekiangensis and P. zhennan. Three species were amplified with ISSR-PCR technique.[Results]The optimized CTAB-SDS and CTAB methods were better for DNA extraction of 3 woods. 7 ISSR primers were screened and their amplifica-tion were stable,clear and high polymorphic. A total of 67 bands were obtained through 7 primers, 43,64.2% of which were polymorphic. DNA fragment size were ranged from 100 bp to 2 000 bp.[Conclusion]These polymorphism bands amplified by the 7 primers can distinguish the woods of 3 species.
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