川芎嗪对庆大霉素耳中毒豚鼠耳蜗组织caspase-3的表达及ABR阈值的影响

摘要

目的：研究川芎嗪（TMP）对庆大霉素（GM）耳中毒豚鼠耳蜗组织caspase-3的表达以及ABR阈值的影响。方法：将健康白色红目豚鼠80只随机分为对照组、GM组、GM+TMP组和TMP组，各组均连续用药10 d，每日测量体重调整药量。在给药前和给药10 d后分别检测ABR阈值，停药处死后应用SABC免疫组化技术检测各组豚鼠耳蜗螺旋神经节、毛细胞以及血管纹caspase-3的表达。结果：（1） GM组耳蜗螺旋神经节细胞、血管纹处和毛细胞平均灰度值与对照组比较，差异有统计学意义（P<0.05）；GM+TMP组耳蜗螺旋神经节细胞和毛细胞的平均灰度值与对照组比较差异无统计学意义（P>0.05），而血管纹细胞的平均灰度值与对照组比较差异有统计学意义（P<0.05）；同时GM+TMP组耳蜗螺旋神经节、毛细胞、血管纹的平均灰度值与GM组比较差异均有统计学意义（P<0.05）。（2）各组用药前ABR阈值差异无统计学意义（P>0.05）。给药10 d后， GM组的ABR阈值明显高于对照组（P＜0.05）；TMP+GM组ABR阈值较GM组明显降低（P<0.05）。结论：TMP可能通过阻断庆大霉素耳中毒豚鼠耳蜗组织中caspase-3的表达从而参与阻止GM对耳蜗损伤的过程，实现对GM性耳聋的保护作用。%Objective:To investigate the effects of Ligustrazine (TMP) on the expression of caspase-3 and ABR threshold in guinea pig cochlea after gentamicin (GM) ototoxicity. Methods:A total of 80 guinea pigs were randomly divided into saline group, GM group, GM+TMP group and TMP group. The duration of treatment was lasted 10 d continuously in all groups. The dose was decided by their weight. The ABR threshold were measured before and after administration of 10 d. SABC immunohistochemical method was used to detect the expression of caspase-3 in the spiral ganglion,cochlea hair cells,and stria vascularis of guinea pigs. Results:(1) The average gray value in the spiral ganglion cells, stria vascularis and hair cells in GM group had significant difference with those in the same position of control group (P<0.05) .The average gray value in the spiral ganglion cells and hair cells in GM+TMP group had no difference with those in the same position of control group (P>0.05), but in stria vascularis (P<0.05) . Meanwhile the average gray value in spiral ganglion, hair cells, stria vascularis in TMP+GM group had significant difference with those in GM group (P<0.05) . (2) The ABR threshold had no significant differences before treatment (P>0.05) . After administration of 10 d, the ABR threshold of GM group was significantly changed (P<0.05) . The ABR thresholds in GM group was significantly higher than that in control group (P<0.05) . The ABR threshold in TMP+GM group was lower than that in GM group (P<0.05) . Conclusion:TMP participates in preventing the process of cochlea injury caused by GM,through blocking the expression of caspase-3 in cochlea tissue of GM ototoxicosis probably,which achieve the protection of GM deafness eventually.