Heat shock protein is a kind of protein which synthesizes rapidly from the bacteria to the higher eukaryote and is synthesized under the response of environmental stress. Because of the molecular weight of small heat shock protein about 20kDa in general, it is called HSP20. In this paper, the HSP20 gene family was identified and analyzed by bioinformatics based on tomato genome database. The results showed that there were at least 43 HSP20 genes in tomato, which were distributed unevenly on 12 chromosomes of tomato. The phylogenetic analysis of tomato, pepper and potato HSP20 showed that there was homologous relationship among them. Sequence analysis of HSP20 promoter sequence revealed a number of cis-acting elements involved in plant glucose metabolism and stress, including SURE and W-box. Based on the analysis of two tomatoes (Heinz1706 and pimpinellifolium) in the RNA-Seq database, it was found that most of the members of the family were highly expressed during fruit development and relatively low in other tissues. These results provide a basis for the identification of the evolutionary origin and function of tomato HSP20 gene.%热激蛋白是从细菌到高等真核生物中普遍存在的一种在受到环境胁迫的响应后迅速合成的蛋白.因为小分子热激蛋白的分子量普遍在20kDa左右,故称为HSP20.借助番茄基因组数据库,利用生物信息学方法对HSP20基因家族进行鉴定及分析,结果表明,番茄中至少含有43个HSP20基因,不均匀的分布在番茄的12条染色体上.通过对茄科植物番茄、辣椒、马铃薯HSP20进行系统进化分析发现,三者存在同源关系.对HSP20基因启动子序列分析发现多个响应植物糖代谢和逆境胁迫的顺式作用元件,包括SURE、W-box等.基于RNA-Seq数据库中2种测序番茄(Heinz1706和pimpinellifolium)的表达分析发现,大多数家族成员在果实发育时期表达量高,在其他组织中的表达量相对较低.这些研究结果为鉴定番茄HSP20基因的进化及其功能奠定了基础.
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